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Contrasted Reactivity to Oxygen Tensions in Frankia sp. Strain CcI3 throughout Nitrogen Fixation and Assimilation
Joint Authors
Tisa, Louis S.
Hezbri, Karima
Ghodhbane-Gtari, Faten
Ktari, Amir
Sbissi, Imed
Gtari, Maher
Beauchemin, Nicholas
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Reconciling the irreconcilable is a primary struggle in aerobic nitrogen-fixing bacteria.
Although nitrogenase is oxygen and reactive oxygen species-labile, oxygen tension is required to sustain respiration.
In the nitrogen-fixing Frankia, various strategies have been developed through evolution to control the respiration and nitrogen-fixation balance.
Here, we assessed the effect of different oxygen tensions on Frankia sp.
strain CcI3 growth, vesicle production, and gene expression under different oxygen tensions.
Both biomass and vesicle production were correlated with elevated oxygen levels under both nitrogen-replete and nitrogen-deficient conditions.
The mRNA levels for the nitrogenase structural genes (nifHDK) were high under hypoxic and hyperoxic conditions compared to oxic conditions.
The mRNA level for the hopanoid biosynthesis genes (sqhC and hpnC) was also elevated under hyperoxic conditions suggesting an increase in the vesicle envelope.
Under nitrogen-deficient conditions, the hup2 mRNA levels increased with hyperoxic environment, while hup1 mRNA levels remained relatively constant.
Taken together, these results indicate that Frankia protects nitrogenase by the use of multiple mechanisms including the vesicle-hopanoid barrier and increased respiratory protection.
American Psychological Association (APA)
Ghodhbane-Gtari, Faten& Hezbri, Karima& Ktari, Amir& Sbissi, Imed& Beauchemin, Nicholas& Gtari, Maher…[et al.]. 2014. Contrasted Reactivity to Oxygen Tensions in Frankia sp. Strain CcI3 throughout Nitrogen Fixation and Assimilation. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-481417
Modern Language Association (MLA)
Ghodhbane-Gtari, Faten…[et al.]. Contrasted Reactivity to Oxygen Tensions in Frankia sp. Strain CcI3 throughout Nitrogen Fixation and Assimilation. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-481417
American Medical Association (AMA)
Ghodhbane-Gtari, Faten& Hezbri, Karima& Ktari, Amir& Sbissi, Imed& Beauchemin, Nicholas& Gtari, Maher…[et al.]. Contrasted Reactivity to Oxygen Tensions in Frankia sp. Strain CcI3 throughout Nitrogen Fixation and Assimilation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-481417
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481417