Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells
Joint Authors
Oliveira, Carla
Teixeira, José A.
Nicolau, Ana
Domingues, Lucília
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Frutalin is the α-D-galactose-binding lectin isolated from breadfruit seeds.
Frutalin was obtained from two different sources: native frutalin was purified from its natural origin, and recombinant frutalin was produced and purified from Pichia pastoris.
This work aimed to study and compare the effect of native and recombinant frutalin on HeLa cervical cancer cells proliferation and apoptosis.
Furthermore, the interaction between frutalin and the HeLa cells was investigated by confocal microscopy.
Despite having different carbohydrate-binding affinities, native and recombinant frutalin showed an identical magnitude of cytotoxicity on HeLa cells growth (IC50~100 μg/mL) and equally induced cell apoptosis.
The interaction studies showed that both lectins were rapidly internalised and targeted to HeLa cell's nucleus.
Altogether, these results indicate that frutalin action is not dependent on its sugar-binding properties.
This study provides important information about the bioactivity of frutalin and contributes to the understanding of the plant lectins cytotoxic activity.
American Psychological Association (APA)
Oliveira, Carla& Nicolau, Ana& Teixeira, José A.& Domingues, Lucília. 2011. Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-481440
Modern Language Association (MLA)
Oliveira, Carla…[et al.]. Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-481440
American Medical Association (AMA)
Oliveira, Carla& Nicolau, Ana& Teixeira, José A.& Domingues, Lucília. Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-481440
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481440