Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells
Joint Authors
Sternad, Karl
Gaiser, Birgit
MacNee, William
Daly, Paul
Drost, Ellen M.
Poland, Craig
Bathoorn, Erik
Source
International Journal of Inflammation
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Introduction.
Gastroesophageal reflux has been associated with chronic inflammatory diseases and may be a cause of airway remodelling.
Aspiration of gastric fluids may cause damage to airway epithelial cells, not only because acidity is toxic to bronchial epithelial cells, but also since it contains digestive enzymes, such as pepsin.
Aim.
To study whether pepsin enhances cytotoxicity and inflammation in airway epithelial cells, and whether this is pH-dependent.
Methods.
Human bronchial epithelial cells were exposed to increasing pepsin concentrations in varying acidic milieus, and cell proliferation and cytokine release were assessed.
Results.
Cell survival was decreased by pepsin exposure depending on its concentration (F=17.4) and pH level of the medium (F=6.5) (both P<0.01).
Pepsin-induced interleukin-8 release was greater at lower pH (F=5.1; P<0.01).
Interleukin-6 induction by pepsin was greater at pH 1.5 compared to pH 2.5 (mean difference 434%; P=0.03).
Conclusion.
Pepsin is cytotoxic to bronchial epithelial cells and induces inflammation in addition to acid alone, dependent on the level of acidity.
Future studies should assess whether chronic aspiration causes airway remodelling in chronic inflammatory lung diseases.
American Psychological Association (APA)
Bathoorn, Erik& Daly, Paul& Gaiser, Birgit& Sternad, Karl& Poland, Craig& MacNee, William…[et al.]. 2011. Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells. International Journal of Inflammation،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-481491
Modern Language Association (MLA)
Bathoorn, Erik…[et al.]. Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells. International Journal of Inflammation No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-481491
American Medical Association (AMA)
Bathoorn, Erik& Daly, Paul& Gaiser, Birgit& Sternad, Karl& Poland, Craig& MacNee, William…[et al.]. Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells. International Journal of Inflammation. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-481491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481491