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Increased Cell-Matrix Adhesion upon Constitutive Activation of Rho Proteins by Cytotoxic Necrotizing Factors from E. coli and Y. pseudotuberculosis
Joint Authors
Kolbe, Tanja
Schmidt, Gudula
Wang, Tianbang
May, Martin
Genth, Harald
Source
Journal of Signal Transduction
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cytotoxic necrotizing factors (CNFs) encompass a class of autotransporter toxins produced by uropathogenic E.
coli (CNF1) or Y.
pseudotuberculosis (CNFy).
CNF toxins deamidate and thereby constitutively activate RhoA, Rac1, and Cdc42.
In this study, the effects of CNF1 on cell-matrix adhesion are analysed using functional cell-adhesion assays.
CNF1 strongly increased cell-matrix binding of suspended Hela cells and decreased the susceptibly of cells to trypsin-induced cell detachment.
Increased cell-matrix binding was also observed upon treatment of Hela cells with isomeric CNFy, that specifically deamidates RhoA.
Increased cell-matrix binding thus appears to depend on RhoA deamidation.
In contrast, increased cell spreading was specifically observed upon CNF1 treatment, suggesting that it rather depended on Rac1/Cdc42 deamidation.
Increased cell-matrix adhesion is further presented to result in reduced cell migration of adherent cells.
In contrast, migration of suspended cells was not affected upon treatment with CNF1 or CNFy.
CNF1 and CNFy thus reduced cell migration specifically under the condition of pre-established cell-matrix adhesion.
American Psychological Association (APA)
May, Martin& Kolbe, Tanja& Wang, Tianbang& Schmidt, Gudula& Genth, Harald. 2012. Increased Cell-Matrix Adhesion upon Constitutive Activation of Rho Proteins by Cytotoxic Necrotizing Factors from E. coli and Y. pseudotuberculosis. Journal of Signal Transduction،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-481558
Modern Language Association (MLA)
May, Martin…[et al.]. Increased Cell-Matrix Adhesion upon Constitutive Activation of Rho Proteins by Cytotoxic Necrotizing Factors from E. coli and Y. pseudotuberculosis. Journal of Signal Transduction No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-481558
American Medical Association (AMA)
May, Martin& Kolbe, Tanja& Wang, Tianbang& Schmidt, Gudula& Genth, Harald. Increased Cell-Matrix Adhesion upon Constitutive Activation of Rho Proteins by Cytotoxic Necrotizing Factors from E. coli and Y. pseudotuberculosis. Journal of Signal Transduction. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-481558
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481558