iTRAQ Quantitative Analysis of Multidrug Resistance Mechanisms in Human Gastric Cancer Cells
Joint Authors
Ren, Hong
Hu, Peng
Zhang, Da-Zhi
Hu, Huai-Dong
Ye, Feng
Li, Sang-Lin
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Multidrug resistance (MDR) is a major obstacle towards a successful treatment of gastric cancer.
However, the mechanisms of MDR are intricate and have not been fully understood.
To elucidate the molecular mechanisms of MDR in gastric cancer, we employed the proteomic approach of isobaric tags for relative and absolute quantification (iTRAQ), followed by LC-MS/MS, using the vincristine-resistant SGC7901/VCR cell line and its parental SGC7901 cell line as a model.
In total, 820 unique proteins were identified and 91 proteins showed to be differentially expressed in SGC7901/VCR compared with SGC7901.
Several differentially expressed proteins were further validated by western blot analysis.
Furthermore, the association of MVP, one of the highly expressed proteins in SGC7901/VCR, with MDR was verified.
Our study is the first application of iTRAQ technology for MDR mechanisms analysis in gastric cancer, and many of the differentially expressed proteins identified have not been linked to MDR in gastric cancer before, which showed the value of this technology in identifying differentially expressed proteins in cancer.
American Psychological Association (APA)
Hu, Huai-Dong& Ye, Feng& Zhang, Da-Zhi& Hu, Peng& Ren, Hong& Li, Sang-Lin. 2010. iTRAQ Quantitative Analysis of Multidrug Resistance Mechanisms in Human Gastric Cancer Cells. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-481649
Modern Language Association (MLA)
Hu, Huai-Dong…[et al.]. iTRAQ Quantitative Analysis of Multidrug Resistance Mechanisms in Human Gastric Cancer Cells. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-481649
American Medical Association (AMA)
Hu, Huai-Dong& Ye, Feng& Zhang, Da-Zhi& Hu, Peng& Ren, Hong& Li, Sang-Lin. iTRAQ Quantitative Analysis of Multidrug Resistance Mechanisms in Human Gastric Cancer Cells. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-481649
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481649