Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels
Joint Authors
Zheng, Li Wu
Ma, Li
Weber, Franz E.
Ehrbar, Martin
Zwahlen, Roger A.
Li, Chun Lei
Cai, Wei Xin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Bioengineered bone substitutes might represent alternatives to autologous bone grafts in medically compromised patients due to reduced operation time and comorbidity.
Due to the lack of an inherent vascular system their dimension is limited to the size of critical bone size defect.
To overcome this shortcoming, the experiment tried to create heterotopic bone around vessels.
In vivo, a two-component fibrin and thrombin gel containing recombinant bone morphogenic protein (rhBMP-2) and transglutamate vascular endothelial growth factor (TG-VEGF) in different ratios, respectively, was injected into a dimensionally stable membrane tube, wrapped around the femoral vessel bundle in twelve New Zealand white rabbits.
Sacrifice occurred eight weeks postoperatively.
Microcomputed tomography of the specimens showed significantly increased bone volume in the rhBMP-2 to TG-VEGF ratio of 10 to 1 group.
Histology showed new bone formation in close proximity to the vessel bundle.
Immunohistochemistry detected increased angiogenesis within the newly formed bone in the rhBMP-2 to TG-VEGF ratios of 3 to 1 and 5 to 1.
Heterotopic bone was engineered in vivo around vessels using different rhBMP-2 and TG-VEGF ratios in a fibrin matrix injected into a dimensionally stable membrane tube which prevented direct contact with skeletal muscles.
American Psychological Association (APA)
Cai, Wei Xin& Zheng, Li Wu& Li, Chun Lei& Ma, Li& Ehrbar, Martin& Weber, Franz E.…[et al.]. 2014. Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-481674
Modern Language Association (MLA)
Cai, Wei Xin…[et al.]. Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-481674
American Medical Association (AMA)
Cai, Wei Xin& Zheng, Li Wu& Li, Chun Lei& Ma, Li& Ehrbar, Martin& Weber, Franz E.…[et al.]. Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-481674
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481674