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Comparative Study of Cyanobacterial and E. coli RNA Polymerases : Misincorporation, Abortive Transcription, and Dependence on Divalent Cations
Joint Authors
Shimamoto, Nobuo
Tanaka, Kan
Imashimizu, Masahiko
Source
Genetics Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
If Mg2+ ion is replaced by Mn2+ ion, RNA polymerase tends to misincorporate noncognate nucleotide, which is thought to be one of the reasons for the toxicity of Mn2+ ion.
Therefore, most cells have Mn2+ ion at low intracellular concentrations, but cyanobacteria need the ion at a millimolar concentration to maintain photosynthetic machinery.
To analyse the mechanism for resistance against the abundant Mn2+ ion, we compared the properties of cyanobacterial and E.
coli RNA polymerases.
The cyanobacterial enzyme showed a lower level of abortive transcription and less misincorporation than the E.
coli enzyme.
Moreover, the cyanobacterial enzyme showed a slower rate of the whole elongation by an order of magnitude, paused more frequently, and cleaved its transcript faster in the absence of NTPs.
In conclusion, cyanobacterial RNA polymerase maintains the fidelity of transcription against Mn2+ ion by deliberate incorporation of a nucleotide at the cost of the elongation rate.
The cyanobacterial and the E.
coli enzymes showed different sensitivities to Mg2+ ion, and the physiological role of the difference is also discussed.
American Psychological Association (APA)
Imashimizu, Masahiko& Tanaka, Kan& Shimamoto, Nobuo. 2011. Comparative Study of Cyanobacterial and E. coli RNA Polymerases : Misincorporation, Abortive Transcription, and Dependence on Divalent Cations. Genetics Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-481787
Modern Language Association (MLA)
Imashimizu, Masahiko…[et al.]. Comparative Study of Cyanobacterial and E. coli RNA Polymerases : Misincorporation, Abortive Transcription, and Dependence on Divalent Cations. Genetics Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-481787
American Medical Association (AMA)
Imashimizu, Masahiko& Tanaka, Kan& Shimamoto, Nobuo. Comparative Study of Cyanobacterial and E. coli RNA Polymerases : Misincorporation, Abortive Transcription, and Dependence on Divalent Cations. Genetics Research International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-481787
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481787