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Standardization of Licorice and TCM Formulations Using Eastern Blot Fingerprinting Analysis
Author
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To prepare the antiglycyrrhizin (GC) monoclonal antibody (MAb), GC was treated with NaIO4 resulting in aldehyde which can be combined with carrier protein.
An antigen conjugate was performed by a matrix-assisted laser desorption/ionization TOF mass spectrometry to determine the hapten numbers in the conjugate.
Anti-GC MAb was prepared from a hybridoma which was fixed from the spleen cells producing anti-GC MAb and the myeloma cells after immunization.
The TCM and licorice extract were developed by TLC and blotted to a polyvinylidene difluoride (PVDF) membrane.
The membrane was treated by NaIO4 and protein, enzyme labeled secondary MAb, and finally substrate was added.
Clear spot appeared on PVDF membrane identifying GC against a background containing large amount of impurities.
In eastern blotting, the GC molecule was divided into two functions.
The aglycone part is recognized as an epitope and the sugar moiety can be combined to membrane.
The specific reactivity of sugar moiety in the GC molecule against anti-GC MAb might be modified by the NaIO4 treatment on the membrane because glycyrrhetic acid 3-O-glucuronide can be stained although the cross-reactivity is only 4.3%.
Eastern blotting for GC can not only apply for the standardization of licorice and TCM, but also it can open for the other bioactive products.
American Psychological Association (APA)
Shoyama, Yukihiro. 2013. Standardization of Licorice and TCM Formulations Using Eastern Blot Fingerprinting Analysis. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-481834
Modern Language Association (MLA)
Shoyama, Yukihiro. Standardization of Licorice and TCM Formulations Using Eastern Blot Fingerprinting Analysis. Journal of Chemistry No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-481834
American Medical Association (AMA)
Shoyama, Yukihiro. Standardization of Licorice and TCM Formulations Using Eastern Blot Fingerprinting Analysis. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-481834
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481834