An Innovative Hyperbaric Hypothermic Machine Perfusion Protects the Liver from Experimental Preservation Injury
Joint Authors
Giannone, Ferdinando A.
Treré, Davide
Bernardi, Mauro
Derenzini, Massimo
Caraceni, Paolo
Solaini, Giancarlo
Grattagliano, Ignazio
Longobardi, Pasquale
Domenicali, Marco
Baracca, Alessandra
Sgarbi, Gianluca
Maggioli, Caterina
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Purpose.
Hypothermic machine perfusion systems seem more effective than the current static storage to prevent cold ischemic liver injury.
Thus, we test an innovative hyperbaric hypothermic machine perfusion (HHMP), which combines hyperbaric oxygenation of the preservation solution and continuous perfusion of the graft.
Methods.
Rat livers were preserved with Celsior solution according to 4 different modalities: normobaric static preservation; hyperbaric static preservation at 2 atmosphere absolute (ATA); normobaric dynamic preservation, with continuous perfusion; hyperbaric dynamic preservation, with continuous perfusion at 2 ATA.
After 24 h cold preservation, we assessed different parameters.
Results.
Compared to baseline, livers preserved with the current static storage showed severe ultrastructural damage, glycogen depletion and an increased oxidative stress.
Normobaric perfused livers showed improved hepatocyte ultrastructure and ameliorated glycogen stores, but they still suffered a significant oxidative damage.
The addition of hyperbaric oxygen produces an extra benefit by improving oxidative injury and by inducing endothelial NO synthase (eNOS) gene expression.
Conclusions.
Preservation by means of the present innovative HHMP reduced the liver injury occurring after the current static cold storage by lowering glycogen depletion and oxidative damage.
Interestingly, only the use of hyperbaric oxygen was associated to a blunted oxidative stress and an increased eNOS gene expression.
American Psychological Association (APA)
Giannone, Ferdinando A.& Treré, Davide& Domenicali, Marco& Grattagliano, Ignazio& Baracca, Alessandra& Sgarbi, Gianluca…[et al.]. 2012. An Innovative Hyperbaric Hypothermic Machine Perfusion Protects the Liver from Experimental Preservation Injury. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-481867
Modern Language Association (MLA)
Giannone, Ferdinando A.…[et al.]. An Innovative Hyperbaric Hypothermic Machine Perfusion Protects the Liver from Experimental Preservation Injury. The Scientific World Journal No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-481867
American Medical Association (AMA)
Giannone, Ferdinando A.& Treré, Davide& Domenicali, Marco& Grattagliano, Ignazio& Baracca, Alessandra& Sgarbi, Gianluca…[et al.]. An Innovative Hyperbaric Hypothermic Machine Perfusion Protects the Liver from Experimental Preservation Injury. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-481867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-481867