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A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design
Joint Authors
Ray, Rina Rani
Karmakar, Moumita
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Information Technology and Computer Science
Abstract EN
The production cost of β-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (SSF).
In the present study, the effect of different production conditions on the yield of β-glucosidase and endoglucanase by Rhizopus oryzae MTCC 9642 from water hyacinth was investigated systematically using response surface methodology.
A Central composite experimental design was applied to optimize the impact of three variables, namely, substrate concentration, pH, and temperature, on enzyme production.
The optimal level of each parameter for maximum enzyme production by the fungus was determined.
Highest activity of endoglucanase of 495 U/mL was achieved at a substrate concentration of 1.23%, pH 7.29, and temperature 29.93°C whereas maximum β-glucosidase activity of 137.32 U/ml was achieved at a substrate concentration of 1.25%, pH 6.66, and temperature 32.09°C.
There was a direct correlation between the levels of enzymatic activities and the substrate concentration of water hyacinth as carbon source.
American Psychological Association (APA)
Karmakar, Moumita& Ray, Rina Rani. 2011. A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-482000
Modern Language Association (MLA)
Karmakar, Moumita& Ray, Rina Rani. A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design. Biotechnology Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-482000
American Medical Association (AMA)
Karmakar, Moumita& Ray, Rina Rani. A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-482000
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482000