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Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients
Joint Authors
Mele, Raffaella
Ravà, Lucilla
Giuliani, Laura
Crinò, Antonino
Altieri, Laura
Di Giovine, Monica
Fierabracci, Alessandra
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Type 1 diabetes (T1D) is an autoimmune disease, in which pancreatic β cells are destroyed in genetically predisposed individuals.
While the direct contribution of autoantibodies to the disease pathogenesis is controversial, it is generally recognised that the mechanism of β cell destruction is mediated by autoreactive T cells that had escaped the thymic selection.
We aimed to design a method to detect circulating CD8+ T cells autoreactive against an epitope of the glutamic acid decarboxylase autoantigen, isoform 65 (GAD65) ex vivo in T1D patients by using HLA class I tetramers.
Low frequencies of GAD65 peptide-specific CD8+ cytotoxic T lymphocytes were detected in peripheral blood lymphocytes (PBMC) of normal controls after GAD65 peptide-specific stimulation.
Conversely, their frequencies were significantly higher than in controls in PBMC of T1D patients after GAD65 peptide stimulation.
These preliminary data are encouraging in order to develop a reliable assay to be employed in large-scale screening studies.
American Psychological Association (APA)
Giuliani, Laura& Mele, Raffaella& Di Giovine, Monica& Altieri, Laura& Crinò, Antonino& Ravà, Lucilla…[et al.]. 2009. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-482091
Modern Language Association (MLA)
Giuliani, Laura…[et al.]. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-482091
American Medical Association (AMA)
Giuliani, Laura& Mele, Raffaella& Di Giovine, Monica& Altieri, Laura& Crinò, Antonino& Ravà, Lucilla…[et al.]. Detection of GAD65 Autoreactive T-Cells by HLA Class I Tetramers in Type 1 Diabetic Patients. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-482091
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482091