The Construction of Chimeric T-Cell Receptor with Spacer Base of Modeling Study of VHH and MUC1 Interaction
Joint Authors
Pirooznia, Nazanin
Taghdir, Majid
Hasannia, Sadegh
Eskandani, Morteza
Rahbarizadeh, Fatemeh
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Adaptive cell immunotherapy with the use of chimeric receptors leads to the best and most specific response against tumors.
Chimeric receptors consist of a signaling fragment, extracellular spacer, costimulating domain, and an antibody.
Antibodies cause immunogenicity; therefore, VHH is a good replacement for ScFv in chimeric receptors.
Since peptide sequences have an influence on chimeric receptors, the effect of peptide domains on each other's conformation were investigated.
CD3Zeta, CD28, VHH and CD8α, and FcgIIα are used as signaling moieties, costimulating domain, antibody, and spacers, respectively.
To investigate the influence of the ligation of spacers on the conformational structure of VHH, models of VHH were constructed.
Molecular dynamics simulation was run to study the influence of the presence of spacers on the conformational changes in the binding sites of VHH.
Root mean square deviation and root mean square fluctuation of critical segments in the binding site showed no noticeable differences with those in the native VHH.
Results from molecular docking revealed that the presence of spacer FcgIIα causes an increasing effect on VHH with MUC1 interaction.
Each of the constructs was transformed into the Jurkat E6.1.
Expression analysis and evaluation of their functions were examined.
The results showed good expression and function.
American Psychological Association (APA)
Pirooznia, Nazanin& Hasannia, Sadegh& Taghdir, Majid& Rahbarizadeh, Fatemeh& Eskandani, Morteza. 2011. The Construction of Chimeric T-Cell Receptor with Spacer Base of Modeling Study of VHH and MUC1 Interaction. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-482179
Modern Language Association (MLA)
Pirooznia, Nazanin…[et al.]. The Construction of Chimeric T-Cell Receptor with Spacer Base of Modeling Study of VHH and MUC1 Interaction. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-482179
American Medical Association (AMA)
Pirooznia, Nazanin& Hasannia, Sadegh& Taghdir, Majid& Rahbarizadeh, Fatemeh& Eskandani, Morteza. The Construction of Chimeric T-Cell Receptor with Spacer Base of Modeling Study of VHH and MUC1 Interaction. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-482179
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482179