Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential
Joint Authors
Sahu, Avinash
Gupta, Pratima
Samant, Kalpana
Source
International Journal of Microbiology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-18
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Eight isolates of cellulose-degrading bacteria (CDB) were isolated from four different invertebrates (termite, snail, caterpillar, and bookworm) by enriching the basal culture medium with filter paper as substrate for cellulose degradation.
To indicate the cellulase activity of the organisms, diameter of clear zone around the colony and hydrolytic value on cellulose Congo Red agar media were measured.
CDB 8 and CDB 10 exhibited the maximum zone of clearance around the colony with diameter of 45 and 50 mm and with the hydrolytic value of 9 and 9.8, respectively.
The enzyme assays for two enzymes, filter paper cellulase (FPC), and cellulase (endoglucanase), were examined by methods recommended by the International Union of Pure and Applied Chemistry (IUPAC).
The extracellular cellulase activities ranged from 0.012 to 0.196 IU/mL for FPC and 0.162 to 0.400 IU/mL for endoglucanase assay.
All the cultures were also further tested for their capacity to degrade filter paper by gravimetric method.
The maximum filter paper degradation percentage was estimated to be 65.7 for CDB 8.
Selected bacterial isolates CDB 2, 7, 8, and 10 were co-cultured with Saccharomyces cerevisiae for simultaneous saccharification and fermentation.
Ethanol production was positively tested after five days of incubation with acidified potassium dichromate.
American Psychological Association (APA)
Gupta, Pratima& Samant, Kalpana& Sahu, Avinash. 2012. Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential. International Journal of Microbiology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-482268
Modern Language Association (MLA)
Gupta, Pratima…[et al.]. Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential. International Journal of Microbiology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-482268
American Medical Association (AMA)
Gupta, Pratima& Samant, Kalpana& Sahu, Avinash. Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential. International Journal of Microbiology. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-482268
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482268