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Electron Beam Irradiation Dose Dependently Damages the Bacillus Spore Coat and Spore Membrane
Joint Authors
Uribe, R. M.
Woolverton, C. J.
Helfinstine, S. L.
Fiester, S. E.
Redfearn, J. C.
Source
International Journal of Microbiology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Effective control of spore-forming bacilli begs suitable physical or chemical methods.
While many spore inactivation techniques have been proven effective, electron beam (EB) irradiation has been frequently chosen to eradicate Bacillus spores.
Despite its widespread use, there are limited data evaluating the effects of EB irradiation on Bacillus spores.
To study this, B.
atrophaeus spores were purified, suspended in sterile, distilled water, and irradiated with EB (up to 20 kGy).
Irradiated spores were found (1) to contain structural damage as observed by electron microscopy, (2) to have spilled cytoplasmic contents as measured by spectroscopy, (3) to have reduced membrane integrity as determined by fluorescence cytometry, and (4) to have fragmented genomic DNA as measured by gel electrophoresis, all in a dose-dependent manner.
Additionally, cytometry data reveal decreased spore size, increased surface alterations, and increased uptake of propidium iodide, with increasing EB dose, suggesting spore coat alterations with membrane damage, prior to loss of spore viability.
The present study suggests that EB irradiation of spores in water results in substantial structural damage of the spore coat and inner membrane, and that, along with DNA fragmentation, results in dose-dependent spore inactivation.
American Psychological Association (APA)
Fiester, S. E.& Helfinstine, S. L.& Redfearn, J. C.& Uribe, R. M.& Woolverton, C. J.. 2012. Electron Beam Irradiation Dose Dependently Damages the Bacillus Spore Coat and Spore Membrane. International Journal of Microbiology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-482337
Modern Language Association (MLA)
Fiester, S. E.…[et al.]. Electron Beam Irradiation Dose Dependently Damages the Bacillus Spore Coat and Spore Membrane. International Journal of Microbiology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-482337
American Medical Association (AMA)
Fiester, S. E.& Helfinstine, S. L.& Redfearn, J. C.& Uribe, R. M.& Woolverton, C. J.. Electron Beam Irradiation Dose Dependently Damages the Bacillus Spore Coat and Spore Membrane. International Journal of Microbiology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-482337
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482337