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Several Affinity Tags Commonly Used in Chromatographic Purification
Joint Authors
Liang, Shufang
Li, Guoshun
Zhao, Xinyu
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Affinity tags have become powerful tools from basic biological research to structural and functional proteomics.
They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes.
Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, Strep II tag, streptavidin-binding peptide (SBP) tag, calmodulin-binding peptide (CBP), glutathione S-transferase (GST), maltose-binding protein (MBP), S-tag, HA tag, and c-Myc tag.
In some cases, a large-size affinity tag, such as GST or MBP, can significantly impact on the structure and biological activity of the fusion partner protein.
So it is usually necessary to excise the tag by protease.
The most commonly used endopeptidases are enterokinase, factor Xa, thrombin, tobacco etch virus, and human rhinovirus 3C protease.
The proteolysis features of these proteases are described in order to provide a general guidance on the proteolytic removal of the affinity tags.
American Psychological Association (APA)
Zhao, Xinyu& Li, Guoshun& Liang, Shufang. 2013. Several Affinity Tags Commonly Used in Chromatographic Purification. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-482476
Modern Language Association (MLA)
Zhao, Xinyu…[et al.]. Several Affinity Tags Commonly Used in Chromatographic Purification. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-482476
American Medical Association (AMA)
Zhao, Xinyu& Li, Guoshun& Liang, Shufang. Several Affinity Tags Commonly Used in Chromatographic Purification. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-482476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482476