Epigenetic Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer
Joint Authors
Kumari, Soni
Singh, Sunita
Pradhan, Satyajit
Pandey, Lakshmi Kant
Das, Mitali
Yadav, Suresh Singh
Prasad, Shyam Babu
Narayan, Gopeshwar
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In the network of chemokine signaling pathways, recent reports have described the SDF-1α/CXCR4 axis and its role in cancer progression and metastasis.
Interestingly, we found downregulation of CXCR4 at both transcript and protein level in cervical cancer cell lines and primary tumors.
We also found CXCR4 promoter hypermethylation in cervical cancer cell lines and primary biopsy samples.
DNA hypomethylating drug 5-AZA-2′-deoxycytidine and histone deacetylase inhibitor Trichostatin A treatments in cell lines reactivate both CXCR4 transcription and protein expression.
Cell adhesion assay demonstrated that autocrine SDF-1α promotes the loss of cell adhesion while paracrine SDF-1α predominantly protects the normal cervical cells from loss of cell adhesion.
Cervical cancer cell line C-33A having increased expression of CXCR4 after TSA treatment showed increased cell adhesion by paracrine source of SDF-1α in comparison to untreated C-33A.
These findings demonstrate the first evidence that epigenetic silencing of CXCR4 makes the cells inefficient to respond to the paracrine source of SDF-1α leading to loss of cell adhesion, one of the key events in metastases and progression of the disease.
Our results provide novel insight of SDF-1α/CXCR4 signaling in tumor microenvironment which may be promising to further delineate molecular mechanism of cervical carcinogenesis.
American Psychological Association (APA)
Yadav, Suresh Singh& Prasad, Shyam Babu& Das, Mitali& Kumari, Soni& Pandey, Lakshmi Kant& Singh, Sunita…[et al.]. 2014. Epigenetic Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-482493
Modern Language Association (MLA)
Yadav, Suresh Singh…[et al.]. Epigenetic Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-482493
American Medical Association (AMA)
Yadav, Suresh Singh& Prasad, Shyam Babu& Das, Mitali& Kumari, Soni& Pandey, Lakshmi Kant& Singh, Sunita…[et al.]. Epigenetic Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-482493
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482493