Application of Phosphoproteomics to Find Targets of Casein Kinase 1 in the Flagellum of Chlamydomonas
Joint Authors
Weisheit, Wolfram
Wagner, Volker
Mittag, Maria
Boesger, Jens
Source
International Journal of Plant Genomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The green biflagellate alga Chlamydomonas reinhardtii serves as model for studying structural and functional features of flagella.
The axoneme of C.
reinhardtii anchors a network of kinases and phosphatases that control motility.
One of them, Casein Kinase 1 (CK1), is known to phosphorylate the Inner Dynein Arm I1 Intermediate Chain 138 (IC138), thereby regulating motility.
CK1 is also involved in regulating the circadian rhythm of phototaxis and is relevant for the formation of flagella.
By a comparative phosphoproteome approach, we determined phosphoproteins in the flagellum that are targets of CK1.
Thereby, we applied the specific CK1 inhibitor CKI-7 that causes significant changes in the flagellum phosphoproteome and reduces the swimming velocity of the cells.
In the CKI-7-treated cells, 14 phosphoproteins were missing compared to the phosphoproteome of untreated cells, including IC138, and four additional phosphoproteins had a reduced number of phosphorylation sites.
Notably, inhibition of CK1 causes also novel phosphorylation events, indicating that it is part of a kinase network.
Among them, Glycogen Synthase Kinase 3 is of special interest, because it is involved in the phosphorylation of key clock components in flies and mammals and in parallel plays an important role in the regulation of assembly in the flagellum.
American Psychological Association (APA)
Boesger, Jens& Wagner, Volker& Weisheit, Wolfram& Mittag, Maria. 2012. Application of Phosphoproteomics to Find Targets of Casein Kinase 1 in the Flagellum of Chlamydomonas. International Journal of Plant Genomics،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-482502
Modern Language Association (MLA)
Boesger, Jens…[et al.]. Application of Phosphoproteomics to Find Targets of Casein Kinase 1 in the Flagellum of Chlamydomonas. International Journal of Plant Genomics No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-482502
American Medical Association (AMA)
Boesger, Jens& Wagner, Volker& Weisheit, Wolfram& Mittag, Maria. Application of Phosphoproteomics to Find Targets of Casein Kinase 1 in the Flagellum of Chlamydomonas. International Journal of Plant Genomics. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-482502
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482502