Overexpression of Delayed Rectifier K+ Channels Promotes In situ Proliferation of Leukocytes in Rat Kidneys with Advanced Chronic Renal Failure
Joint Authors
Ejima, Yutaka
Matsubara, Mitsunobu
Toyama, Hiroaki
Kurosawa, Shin
Endo, Yasuhiro
Kazama, Itsuro
Maruyama, Yoshio
Source
International Journal of Nephrology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Leukocytes, such as lymphocytes and macrophages, predominantly express delayed rectifier K+ channels (Kv1.3), and the channels play crucial roles in the activation and proliferation of the cells.
Since lymphocytes are activated in patients with end-stage renal disease (ESRD), the channels expressed in those cells would contribute to the progression of renal fibrosis in advanced-stage chronic renal failure (CRF).
In the present study, using a rat model with advanced CRF that underwent 5/6 nephrectomy followed by a 14-week recovery period, we examined the histopathological features of the kidneys and the leukocyte expression of Kv1.3-channels and cell cycle markers.
Age-matched sham-operated rats were used as controls.
In the cortical interstitium of advanced CRF rat kidneys, leukocytes proliferated in situ and overexpressed Kv1.3 channel protein in their cytoplasm.
Treatment with margatoxin, a selective Kv1.3-channel inhibitor, significantly suppressed the number of leukocytes and the progression of renal fibrosis with a significant decrease in the cortical cell cycle marker expression.
This study demonstrated for the first time that the number of leukocytes was dramatically increased in rat kidneys with advanced CRF.
The overexpression of Kv1.3 channels in the leukocytes was thought to contribute to the progression of renal fibrosis by stimulating cell cycling and promoting cellular proliferation.
American Psychological Association (APA)
Kazama, Itsuro& Maruyama, Yoshio& Endo, Yasuhiro& Toyama, Hiroaki& Ejima, Yutaka& Matsubara, Mitsunobu…[et al.]. 2012. Overexpression of Delayed Rectifier K+ Channels Promotes In situ Proliferation of Leukocytes in Rat Kidneys with Advanced Chronic Renal Failure. International Journal of Nephrology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-482513
Modern Language Association (MLA)
Kazama, Itsuro…[et al.]. Overexpression of Delayed Rectifier K+ Channels Promotes In situ Proliferation of Leukocytes in Rat Kidneys with Advanced Chronic Renal Failure. International Journal of Nephrology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-482513
American Medical Association (AMA)
Kazama, Itsuro& Maruyama, Yoshio& Endo, Yasuhiro& Toyama, Hiroaki& Ejima, Yutaka& Matsubara, Mitsunobu…[et al.]. Overexpression of Delayed Rectifier K+ Channels Promotes In situ Proliferation of Leukocytes in Rat Kidneys with Advanced Chronic Renal Failure. International Journal of Nephrology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-482513
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482513