Approaching Biomarkers of Membranous Nephropathy from a Murine Model to Human Disease
Joint Authors
Huang, Ching-Feng
Chen, Chun-Chi
Sytwu, Huey-Kang
Wu, Chia-Chao
Chu, Pauling
Lu, Kuo-Cheng
Lin, Yuh-Feng
Chen, Jin-Shuen
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Membranous glomerulonephropathy (MN) is the most prevalent cause of nephrotic syndrome in adult humans.
However, the specific biomarkers of MN have not been fully elucidated.
We examined the alterations in gene expression associated with the development of MN.
Methods.
Murine MN was induced by cationic bovine serum albumin (cBSA).
After full-blown MN, cDNA microarray analysis was performed to identify gene expression changes, and highly expressed genes were evaluated as markers both in mice and human kidney samples.
Results.
MN mice revealed clinical proteinuria and the characteristic diffuse thickening of the glomerular basement membrane.
There were 175 genes with significantly different expressions in the MN kidneys compared with the normal kidneys.
Four genes, metallothionein-1 (Mt1), cathepsin D (CtsD), lymphocyte 6 antigen complex (Ly6), and laminin receptor-1 (Lamr1), were chosen and quantified.
Mt1 was detected mainly in tubules, Lamr1 was highly expressed in glomeruli, and CtsD was detected both in tubules and glomeruli.
The high expressions of Lamr1 and CtsD were also confirmed in human kidney biopsies.
Conclusion.
The murine MN model resembled the clinical and pathological features of human MN and may provide a tool for investigating MN.
Applying cDNA microarray analysis may help to identify biomarkers for human MN.
American Psychological Association (APA)
Wu, Chia-Chao& Chen, Jin-Shuen& Huang, Ching-Feng& Chen, Chun-Chi& Lu, Kuo-Cheng& Chu, Pauling…[et al.]. 2010. Approaching Biomarkers of Membranous Nephropathy from a Murine Model to Human Disease. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-482545
Modern Language Association (MLA)
Wu, Chia-Chao…[et al.]. Approaching Biomarkers of Membranous Nephropathy from a Murine Model to Human Disease. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-482545
American Medical Association (AMA)
Wu, Chia-Chao& Chen, Jin-Shuen& Huang, Ching-Feng& Chen, Chun-Chi& Lu, Kuo-Cheng& Chu, Pauling…[et al.]. Approaching Biomarkers of Membranous Nephropathy from a Murine Model to Human Disease. Journal of Biomedicine and Biotechnology. 2010. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-482545
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482545