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Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells : A Novel Therapy for Insulin-Dependent Diabetes Mellitus
Joint Authors
Vanikar, Aruna V.
Trivedi, Hargovind L.
Thakkar, U. G.
Dave, S. D.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-20
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Aims.
Insulin dependent diabetes mellitus (IDDM) is believed to be an autoimmune disorder with disturbed glucose/insulin metabolism, requiring life-long insulin replacement therapy (IRT), 30% of patients develop end-organ failure.
We present our experience of cotransplantation of adipose tissue derived insulin-secreting mesenchymal stem cells (IS-AD-MSC) and cultured bone marrow (CBM) as IRT for these patients.
Methods.
This was a prospective open-labeled clinical trial to test efficacy and safety of IS-AD-MSC+CBM co-transplantation to treat IDDM, approved by the institutional review board after informed consent in 11 (males : females: 7 : 4) patients with 1–24-year disease duration, in age group: 13–43 years, on mean values of exogenous insulin requirement of 1.14 units/kg BW/day, glycosylated hemoglobin (Hb1Ac): 8.47%, and c-peptide levels: 0.1 ng/mL.
Intraportal infusion of xenogeneic-free IS-AD-MSC from living donors, subjected to defined culture conditions and phenotypically differentiated to insulin-secreting cells, with mean quantum: 1.5 mL, expressing Pax-6, Isl-1, and pdx-1, cell counts: 2.1×103/μL, CD45−/90+/73+:40/30.1%, C-Peptide level:1.8 ng/mL, and insulin level: 339.3 IU/mL with CBM mean quantum: 96.3 mL and cell counts: 28.1×103/μL, CD45−/34+:0.62%, was carried out.
Results.
All were successfully transplanted without any untoward effect.
Over mean followup of 23 months, they had a decreased mean exogenous insulin requirement to 0.63 units/kgBW/day, Hb1Ac to 7.39%, raised serum c-peptide levels to 0.38 ng/mL, and became free of diabetic ketoacidosis events with mean 2.5 Kg weight gain on normal vegetarian diet and physical activities.
Conclusion.
This is the first report of treating IDDM with insulin-secreting-AD-MSC+CBM safely and effectively with relatively simple techniques.
American Psychological Association (APA)
Vanikar, Aruna V.& Dave, S. D.& Thakkar, U. G.& Trivedi, Hargovind L.. 2010. Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells : A Novel Therapy for Insulin-Dependent Diabetes Mellitus. Stem Cells International،Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-482582
Modern Language Association (MLA)
Vanikar, Aruna V.…[et al.]. Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells : A Novel Therapy for Insulin-Dependent Diabetes Mellitus. Stem Cells International No. 2010 (2010), pp.1-5.
https://search.emarefa.net/detail/BIM-482582
American Medical Association (AMA)
Vanikar, Aruna V.& Dave, S. D.& Thakkar, U. G.& Trivedi, Hargovind L.. Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells : A Novel Therapy for Insulin-Dependent Diabetes Mellitus. Stem Cells International. 2010. Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-482582
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482582