The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats
Joint Authors
Wang, Ting Yu
Wu, Pui Kei
Liu, Liang
Leung, Elaine Lai-Han
Zhou, Hua
Hsiao, Wen-Luan Wendy
Wong, Yuen Fan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-27
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Qingfu Guanjieshu (QFGJS) is an herbal preparation for treating rheumatoid arthritis (RA).
Previous studies revealed that QFGJS significantly inhibited experimental arthritis and acute inflammation, accompanied by reduction of proinflammatory cytokines and elevation of anti-inflammatory cytokines.
This study aims to identify the targeted proteins and predict the proteomic network associated with the drug action of QFGJS by using 2D gel and MALDI-TOF-MS/MS techniques.
Thirty female Wistar rats were evenly grouped as normal and vehicle- and QFGJS-treated CIA rats.
The antiarthritic effect of QFGJS was examined with a 19-day treatment course, and the knee synovial tissues of animals from each group were obtained for 2D gel and MALDI-TOF-MS/MS analysis.
Results showed that QFGJS significantly ameliorated collagen II-induced arthritis when administrated at 2.8 g/kg body weight for 19 days.
2D gel image analysis revealed 89 differentially expressed proteins in the synovial tissues among the normal and vehicle- and QFGJS-treated CIA rats from over 1000 proteins of which 63 proteins were identified by MALDI-TOF-MS/MS analysis, and 32 proteins were included for classification of functions using Gene Ontology (GO) method.
Finally, 14 proteins were analyzed using bioinformatics, and a predicted proteomic network related to the anti-arthritic effect of QFGJS was established, and Pgk1 plays a central role.
American Psychological Association (APA)
Wang, Ting Yu& Zhou, Hua& Wong, Yuen Fan& Wu, Pui Kei& Hsiao, Wen-Luan Wendy& Leung, Elaine Lai-Han…[et al.]. 2013. The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-482592
Modern Language Association (MLA)
Wang, Ting Yu…[et al.]. The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-482592
American Medical Association (AMA)
Wang, Ting Yu& Zhou, Hua& Wong, Yuen Fan& Wu, Pui Kei& Hsiao, Wen-Luan Wendy& Leung, Elaine Lai-Han…[et al.]. The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-482592
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482592