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The Molecular Mechanisms of Tanshinone IIA on the Apoptosis and Arrest of Human Esophageal Carcinoma Cells
Joint Authors
Feng, Jian-Guo
Wang, Jiang-Feng
Zhang, Bei-Bei
Han, Jing
Mao, Wei-Min
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To explore the possible mechanisms of Tanshinone IIA (TanIIA) on esophageal carcinoma cell lines.
Methods.
Two human esophageal carcinoma cell lines (EC-1 cells and ECa-109 cells) were treated with different concentrations of TanIIA.
Cell proliferation was measured by CCK-8, colony-forming efficiency was calculated, cell cycle and apoptosis were measured, and changes in cell cycle- and apoptosis-related gene expression were measured by Western blotting.
Results.
The CCK-8 and colony formation assay indicated that TanIIA inhibited the cell proliferation of human esophageal cancer cells (IC50 below 1 μg/mL) at 48 h.
Hoechst 33258 and flow cytometry showed that TanIIA induced apoptosis in both esophageal cancer cell lines.
Flow cytometry showed that TanIIA arrested cell cycle in S phase and G2/M phase.
Western blotting analysis showed that Akt1 and its phosphorylation were inhibited, the Bax/Bcl-2 ratio increased, and both caspase-9 and caspase-3 were activated after treatment with 1.3 μg/mL TanIIA at 48 h.
Meanwhile, p53 and p21 protein levels increased, whereas cyclin B1, CDC2, and CDC2 phosphorylation were inhibited.
Conclusion.
TanIIA inhibits the growth of esophageal cancer cells and induces apoptosis in a time-dependent and concentration-dependent manner, possibly by affecting cell cycle- and apoptosis-related signaling pathways.
American Psychological Association (APA)
Wang, Jiang-Feng& Feng, Jian-Guo& Han, Jing& Zhang, Bei-Bei& Mao, Wei-Min. 2014. The Molecular Mechanisms of Tanshinone IIA on the Apoptosis and Arrest of Human Esophageal Carcinoma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-482615
Modern Language Association (MLA)
Wang, Jiang-Feng…[et al.]. The Molecular Mechanisms of Tanshinone IIA on the Apoptosis and Arrest of Human Esophageal Carcinoma Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-482615
American Medical Association (AMA)
Wang, Jiang-Feng& Feng, Jian-Guo& Han, Jing& Zhang, Bei-Bei& Mao, Wei-Min. The Molecular Mechanisms of Tanshinone IIA on the Apoptosis and Arrest of Human Esophageal Carcinoma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-482615
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482615