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Detection of Intranasally Delivered Bone Marrow-Derived Mesenchymal Stromal Cells in the Lesioned Mouse Brain : A Cautionary Report
Joint Authors
Cohen, Bruce M.
Damez-Werno, Diane
Chartoff, Elena H.
Kaufmann, Daniel E.
Hassinger, Linda
McPhie, Donna
Peterson, Jesse
Sonntag, Kai C.
Cataldo, Anne M.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Bone marrow-derived mesenchymal stromal cells (MSCs) hold promise for autologous treatment of neuropathologies.
Intranasal delivery is relatively noninvasive and has recently been reported to result in transport of MSCs to the brain.
However, the ability of MSCs to migrate from nasal passages to sites of neuropathology and ultimately survive has not been fully examined.
In this paper, we harvested MSCs from transgenic mice expressing enhanced green fluorescent protein (cells hereafter referred to as MSC-EGFP) and delivered them intranasally to wild-type mice sustaining mechanical lesions in the striatum.
Using fluorescent, colorimetric, and ultrastructural detection methods, GFP-expressing cells were undetectable in the brain from 3 hours to 2 months after MSC delivery.
However, bright autofluorescence that strongly resembled emission from GFP was observed in the olfactory bulb and striatum of lesioned control and MSC-EGFP-treated mice.
In a control experiment, we directly implanted MSC-EGFPs into the mouse striatum and detected robust GFP expression 1 and 7 days after implantation.
These findings suggest that—under our conditions—intranasally delivered MSC-EGFPs do not survive or migrate in the brain.
Furthermore, our observations highlight the necessity of including appropriate controls when working with GFP as a cellular marker.
American Psychological Association (APA)
Chartoff, Elena H.& Damez-Werno, Diane& Sonntag, Kai C.& Hassinger, Linda& Kaufmann, Daniel E.& Peterson, Jesse…[et al.]. 2011. Detection of Intranasally Delivered Bone Marrow-Derived Mesenchymal Stromal Cells in the Lesioned Mouse Brain : A Cautionary Report. Stem Cells International،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-482963
Modern Language Association (MLA)
Chartoff, Elena H.…[et al.]. Detection of Intranasally Delivered Bone Marrow-Derived Mesenchymal Stromal Cells in the Lesioned Mouse Brain : A Cautionary Report. Stem Cells International No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-482963
American Medical Association (AMA)
Chartoff, Elena H.& Damez-Werno, Diane& Sonntag, Kai C.& Hassinger, Linda& Kaufmann, Daniel E.& Peterson, Jesse…[et al.]. Detection of Intranasally Delivered Bone Marrow-Derived Mesenchymal Stromal Cells in the Lesioned Mouse Brain : A Cautionary Report. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-482963
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482963