Lung-Derived Mediators Induce Cytokine Production in Downstream Organs via an NF-κB-Dependent Mechanism
Joint Authors
McCaig, L.
Patterson, E. K.
Ramic, N.
Veldhuizen, R. A. W.
Lewis, J. F.
Cepinskas, G.
Yamashita, C. M.
Yao, L. J.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In the setting of acute lung injury, levels of circulating inflammatory mediators have been correlated with adverse outcomes.
Previous studies have demonstrated that injured, mechanically ventilated lungs represent the origin of the host inflammatory response; however, mechanisms which perpetuate systemic inflammation remain uncharacterized.
We hypothesized that lung-derived mediators generated by mechanical ventilation (MV) are amplified by peripheral organs in a “feed forward” mechanism of systemic inflammation.
Herein, lung-derived mediators were collected from 129X1/SVJ mice after 2 hours of MV while connected to the isolated perfused mouse lung model setup.
Exposure of liver endothelial cells to lung-derived mediators resulted in a significant increase in G-CSF, IL-6, CXCL-1, CXCL-2, and MCP-1 production compared to noncirculated control perfusate media (P<0.05).
Furthermore, inhibition of the NF-κB pathway significantly mitigated this response.
Changes in gene transcription were confirmed using qPCR for IL-6, CXCL-1, and CXCL-2.
Additionally, liver tissue obtained from mice subjected to 2 hours of in vivo MV demonstrated significant increases in hepatic gene transcription of IL-6, CXCL-1, and CXCL-2 compared to nonventilated controls.
Collectively, this data demonstrates that lung-derived mediators, generated in the setting of MV, are amplified by downstream organs in a feed forward mechanism of systemic inflammation.
American Psychological Association (APA)
Patterson, E. K.& Yao, L. J.& Ramic, N.& Lewis, J. F.& Cepinskas, G.& McCaig, L.…[et al.]. 2013. Lung-Derived Mediators Induce Cytokine Production in Downstream Organs via an NF-κB-Dependent Mechanism. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-482983
Modern Language Association (MLA)
Patterson, E. K.…[et al.]. Lung-Derived Mediators Induce Cytokine Production in Downstream Organs via an NF-κB-Dependent Mechanism. Mediators of Inflammation No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-482983
American Medical Association (AMA)
Patterson, E. K.& Yao, L. J.& Ramic, N.& Lewis, J. F.& Cepinskas, G.& McCaig, L.…[et al.]. Lung-Derived Mediators Induce Cytokine Production in Downstream Organs via an NF-κB-Dependent Mechanism. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-482983
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-482983