Interaction between Calcineurin and Ca2+Calmodulin Kinase-II in Modulating Cellular Functions
Joint Authors
Kubokawa, Manabu
Komagiri, You
Nakamura, Kazuyoshi
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-30
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Roles of calcineurin (CaN), a Ca2+/calmodulin- (CaM-) dependent protein phosphatase, and Ca2+/CaM-dependent protein kinase-II (CaMKII) in modulating K+ channel activity and the intracellular Ca2+ concentration ([Ca2+]i) have been investigated in renal tubule epithelial cells.
The channel current through the cell membrane was recorded with the patch-clamp technique, and [Ca2+]i was monitored using fura-2 imaging.
We found that a CaN-inhibitor, cyclosporin A (CyA), lowered the K+ channel activity and elevated [Ca2+]i, suggesting that CyA closes K+ channels and opens Ca2+-release channels of the cytosolic Ca2+-store.
Moreover, both of these responses were blocked by KN-62, an inhibitor of CaMKII.
It is suggested that the CyA-mediated response results from the activation of CaMKII.
Indeed, Western blot analysis revealed that CyA increased phospho-CaMKII, an active form of CaMKII.
These findings suggest that CaN-dependent dephosphorylation inhibits CaMKII-mediated phosphorylation, and the inhibition of CaN increases phospho-CaMKII, which results in the stimulation of CaMKII-dependent cellular actions.
American Psychological Association (APA)
Kubokawa, Manabu& Nakamura, Kazuyoshi& Komagiri, You. 2011. Interaction between Calcineurin and Ca2+Calmodulin Kinase-II in Modulating Cellular Functions. Enzyme Research،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-483031
Modern Language Association (MLA)
Kubokawa, Manabu…[et al.]. Interaction between Calcineurin and Ca2+Calmodulin Kinase-II in Modulating Cellular Functions. Enzyme Research No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-483031
American Medical Association (AMA)
Kubokawa, Manabu& Nakamura, Kazuyoshi& Komagiri, You. Interaction between Calcineurin and Ca2+Calmodulin Kinase-II in Modulating Cellular Functions. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-483031
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483031