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Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease
Joint Authors
Leclercq, Sophie Yvette
Vieira, Fernanda Silva
Guerra de Andrade, Milton Hércules
Paro, Marina de Oliveira
Castro-Borges, William
Namen-Lopes, Maria Sueli Silva
Velloso-Rodrigues, Cibele
Ferreira, Cyntia Silva
de Santana, Marcos Aurélio
Source
International Journal of Peptides
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Von Willebrand disease (VWD) is an inherited hemorrhagic disorder promoted by either quantitative or qualitative defects of the von Willebrand factor (VWF).
The disease represents the most common human coagulopathy afflicting 1.3% of the population.
Qualitative defects are subdivided into four subtypes and classified according to the molecular dysfunction of the VWF.
The differential diagnosis of the VWD is a difficult task, relying on a panel of tests aimed to assess the plasma levels and function of the VWF.
Here, we propose biochemical approaches for the identification of structural variants of the VWF.
A bioinformatic analysis was conducted to design seven peptides among which three were representatives of specific amino acid sequences belonging to normal VWF and four encompassed sequences found in the most common VWD subtype 2B.
These peptides were used to immunize mice, after which, peptide-specific immunoglobulins were purified.
This resulted in four Ig preparations capable of detecting alterations in the subtype 2B VWD plus additional three antibody fractions targeting the normal VWF.
The panel of antibodies could serve many applications among them (1) assessment of VWF: antigen interaction, (2) VWF multimer analysis, and (3) production of monoclonal antibodies against VWF for therapeutic purposes as in thrombotic thrombocytopenic purpura.
American Psychological Association (APA)
Paro, Marina de Oliveira& Ferreira, Cyntia Silva& Vieira, Fernanda Silva& de Santana, Marcos Aurélio& Castro-Borges, William& Namen-Lopes, Maria Sueli Silva…[et al.]. 2013. Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease. International Journal of Peptides،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-483222
Modern Language Association (MLA)
Paro, Marina de Oliveira…[et al.]. Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease. International Journal of Peptides No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-483222
American Medical Association (AMA)
Paro, Marina de Oliveira& Ferreira, Cyntia Silva& Vieira, Fernanda Silva& de Santana, Marcos Aurélio& Castro-Borges, William& Namen-Lopes, Maria Sueli Silva…[et al.]. Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease. International Journal of Peptides. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-483222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483222