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Molecular Characterisation of Bacterial Community Structure along the Intestinal Tract of Zebrafish (Danio rerio) : A Pilot Study
Joint Authors
Lan, Chuan-Ching
Love, Donald R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The bacterial composition along the intestinal tract of Danio rerio was investigated by cultivation-independent analysis of the 16S rRNA gene.
Clone libraries were constructed for three compartments of the intestinal tract of individual fish.
566 individual clones were differentiated by amplified 16S rRNA gene restriction analysis (ARDRA), and clone representatives from each operational taxonomic unit (OTU) were sequenced.
As reported in other studies, we found that Proteobacteria was the most prominent phylum among clone libraries from different fish.
Data generated from this pilot study indicated some compositional differences in bacterial communities.
Two dominant classes, Gammaproteobacteria and Bacilli, displayed different levels of abundance in different compartments; Gammaproteobacteria increased along the intestinal tract, while Bacilli decreased its abundance along the proximal-distal axis.
Less obvious spatial patterns were observed for other classes.
In general, bacterial diversity in the intestinal bulb was greater than that in the posterior intestine.
Interindividual differences in bacterial diversity and composition were also noted in this study.
American Psychological Association (APA)
Lan, Chuan-Ching& Love, Donald R.. 2012. Molecular Characterisation of Bacterial Community Structure along the Intestinal Tract of Zebrafish (Danio rerio) : A Pilot Study. ISRN Microbiology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-483227
Modern Language Association (MLA)
Lan, Chuan-Ching& Love, Donald R.. Molecular Characterisation of Bacterial Community Structure along the Intestinal Tract of Zebrafish (Danio rerio) : A Pilot Study. ISRN Microbiology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-483227
American Medical Association (AMA)
Lan, Chuan-Ching& Love, Donald R.. Molecular Characterisation of Bacterial Community Structure along the Intestinal Tract of Zebrafish (Danio rerio) : A Pilot Study. ISRN Microbiology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-483227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483227