Inhibition of HIV Replication by Cyclic and Hairpin PNAs Targeting the HIV-1 TAR RNA Loop
Joint Authors
Manvar, Dinesh
Patino, Nadia
Upert, Gregory
Upadhyay, Alok
Pandey, Virendra N.
Pandey, Nootan
Di Giorgio, Audrey
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Human immunodeficiency virus-1 (HIV-1) replication and gene expression entails specific interaction of the viral protein Tat with its transactivation responsive element (TAR), to form a highly stable stem-bulge-loop structure.
Previously, we described triphenylphosphonium (TPP) cation-based vectors that efficiently deliver nucleotide analogs (PNAs) into the cytoplasm of cells.
In particular, we showed that the TPP conjugate of a linear 16-mer PNA targeting the apical stem-loop region of TAR impedes Tat-mediated transactivation of the HIV-1 LTR in vitro and also in cell culture systems.
In this communication, we conjugated TPP to cyclic and hairpin PNAs targeting the loop region of HIV-1 TAR and evaluated their antiviral efficacy in a cell culture system.
We found that TPP-cyclic PNAs containing only 8 residues, showed higher antiviral potency compared to hairpin PNAs of 12 or 16 residues.
We further noted that the TPP-conjugates of the 8-mer cyclic PNA as well as the 16-mer linear PNA displayed similar antiviral efficacy.
However, cyclic PNAs were shown to be highly specific to their target sequences.
This communication emphasizes on the importance of small constrained cyclic PNAs over both linear and hairpin structures for targeting biologically relevant RNA hairpins.
American Psychological Association (APA)
Upert, Gregory& Di Giorgio, Audrey& Upadhyay, Alok& Manvar, Dinesh& Pandey, Nootan& Pandey, Virendra N.…[et al.]. 2012. Inhibition of HIV Replication by Cyclic and Hairpin PNAs Targeting the HIV-1 TAR RNA Loop. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-483302
Modern Language Association (MLA)
Upert, Gregory…[et al.]. Inhibition of HIV Replication by Cyclic and Hairpin PNAs Targeting the HIV-1 TAR RNA Loop. Journal of Nucleic Acids No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-483302
American Medical Association (AMA)
Upert, Gregory& Di Giorgio, Audrey& Upadhyay, Alok& Manvar, Dinesh& Pandey, Nootan& Pandey, Virendra N.…[et al.]. Inhibition of HIV Replication by Cyclic and Hairpin PNAs Targeting the HIV-1 TAR RNA Loop. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-483302
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483302