Development of a Cell-Based Functional Assay for the Detection of Clostridium botulinum Neurotoxin Types A and E
Joint Authors
Sharma, Shashi K.
Muruvanda, Tim
Basavanna, Uma
Brown, Eric W.
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
The standard procedure for definitive detection of BoNT-producing Clostridia is a culture method combined with neurotoxin detection using a standard mouse bioassay (MBA).
The mouse bioassay is highly sensitive and specific, but it is expensive and time-consuming, and there are ethical concerns due to use of laboratory animals.
Cell-based assays provide an alternative to the MBA in screening for BoNT-producing Clostridia.
Here, we describe a cell-based assay utilizing a fluorescence reporter construct expressed in a neuronal cell model to study toxin activity in situ.
Our data indicates that the assay can detect as little as 100 pM BoNT/A activity within living cells, and the assay is currently being evaluated for the analysis of BoNT in food matrices.
Among available in vitro assays, we believe that cell-based assays are widely applicable in high-throughput screenings and have the potential to at least reduce and refine animal assays if not replace it.
American Psychological Association (APA)
Basavanna, Uma& Muruvanda, Tim& Brown, Eric W.& Sharma, Shashi K.. 2013. Development of a Cell-Based Functional Assay for the Detection of Clostridium botulinum Neurotoxin Types A and E. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-483518
Modern Language Association (MLA)
Basavanna, Uma…[et al.]. Development of a Cell-Based Functional Assay for the Detection of Clostridium botulinum Neurotoxin Types A and E. International Journal of Microbiology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-483518
American Medical Association (AMA)
Basavanna, Uma& Muruvanda, Tim& Brown, Eric W.& Sharma, Shashi K.. Development of a Cell-Based Functional Assay for the Detection of Clostridium botulinum Neurotoxin Types A and E. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-483518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483518