Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein
Joint Authors
Jain, Chakresh K.
Kumar, Kapila
Gulati, Sahil
Gupta, Amita
Chaudhary, Vijay K.
Rana, Jyoti
Gabrani, Reema
Gupta, Sanjay
Rajasekharan, Sreejith
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The nucleocapsid (N) protein of Chandipura virus (CHPV) plays a crucial role in viral life cycle, besides being an important structural component of the virion through proper organization of its interactions with other viral proteins.
In a recent study, the authors had mapped the associations among CHPV proteins and shown that N protein interacts with four of the viral proteins: N, phosphoprotein (P), matrix protein (M), and glycoprotein (G).
The present study aimed to distinguish the regions of CHPV N protein responsible for its interactions with other viral proteins.
In this direction, we have generated the structure of CHPV N protein by homology modeling using SWISS-MODEL workspace and Accelrys Discovery Studio client 2.55 and mapped the domains of N protein using PiSQRD.
The interactions of N protein fragments with other proteins were determined by ZDOCK rigid-body docking method and validated by yeast two-hybrid and ELISA.
The study revealed a unique binding site, comprising of amino acids 1–30 at the N terminus of the nucleocapsid protein (N1) that is instrumental in its interactions with N, P, M, and G proteins.
It was also observed that N2 associates with N and G proteins while N3 interacts with N, P, and M proteins.
American Psychological Association (APA)
Kumar, Kapila& Rajasekharan, Sreejith& Gulati, Sahil& Rana, Jyoti& Gabrani, Reema& Jain, Chakresh K.…[et al.]. 2013. Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein. Advances in Virology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-483617
Modern Language Association (MLA)
Kumar, Kapila…[et al.]. Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein. Advances in Virology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-483617
American Medical Association (AMA)
Kumar, Kapila& Rajasekharan, Sreejith& Gulati, Sahil& Rana, Jyoti& Gabrani, Reema& Jain, Chakresh K.…[et al.]. Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein. Advances in Virology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-483617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483617