Applicability of the Filter Paper Technique for Detection of Antifilarial IgG4 Antibodies Using the Bm14 Filariasis CELISA

Joint Authors

Joseph, Hayley M.
Melrose, Wayne

Source

Journal of Parasitology Research

Issue

Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2010-02-10

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Zoology
Diseases

Abstract EN

Demonstration of successful elimination of lymphatic filariasis (LF) in endemic countries requires sensitive diagnostics for accurate definitions of endpoints and future surveillance.

There has been interest in complementing available diagnostics with antibody serology testing in children, since negative serology would correspond with cessation of LF transmission.

The Filariasis CELISA detects antifilarial IgG4 and has favourable results with serum samples but field application requires an easier sampling method.

Ninety-four paired plasma and filter paper samples were assayed with promising results.

The filter paper method resulted in a sensitivity of 92% and a specificity of 77% when compared to the paired plasma.

One hundred and one filter paper samples were assessed for storage effects.

Following 10-month storage at −20°C there was a significant reduction in reactivity (P<.001).

Overall the results indicated that filter paper sampling would be a favourable sensitive and specific alternative for blood collection in surveys.

American Psychological Association (APA)

Joseph, Hayley M.& Melrose, Wayne. 2010. Applicability of the Filter Paper Technique for Detection of Antifilarial IgG4 Antibodies Using the Bm14 Filariasis CELISA. Journal of Parasitology Research،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-483649

Modern Language Association (MLA)

Joseph, Hayley M.& Melrose, Wayne. Applicability of the Filter Paper Technique for Detection of Antifilarial IgG4 Antibodies Using the Bm14 Filariasis CELISA. Journal of Parasitology Research No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-483649

American Medical Association (AMA)

Joseph, Hayley M.& Melrose, Wayne. Applicability of the Filter Paper Technique for Detection of Antifilarial IgG4 Antibodies Using the Bm14 Filariasis CELISA. Journal of Parasitology Research. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-483649

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-483649