Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii
Joint Authors
Kaur, Punit
Sharma, Sujata
Sinha, Mau
Sikarwar, Juhi
Kaushik, Sanket
Singh, Tej P.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-11
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Acinetobacter baumannii is a multidrug resistant pathogenic bacteria associated with hospital acquired infections.
This bacterium possesses a variety of resistance mechanisms which makes it more difficult to control the bacterium with conventional drugs, and, so far no effective drug treatment is available against it.
Nucleoside diphosphate kinase is an important enzyme, which maintains the total nucleotide triphosphate pool inside the cell by the transfer of γ-phosphate from NTPs to NDPs.
The role of nucleoside diphosphate kinase (Ndk) has also been observed in pathogenesis in other organisms.
However, intensive studies are needed to decipher its other putative roles in Acinetobacter baumannii.
In the present study, we have successfully cloned the gene encoding Ndk and achieved overexpression in bacterial host BL-21 (DE3).
The overexpressed protein is further purified by nickel-nitrilotriacetic acid (Ni-NTA) chromatography.
American Psychological Association (APA)
Sikarwar, Juhi& Kaushik, Sanket& Sinha, Mau& Kaur, Punit& Sharma, Sujata& Singh, Tej P.. 2013. Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii. Enzyme Research،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-483858
Modern Language Association (MLA)
Sikarwar, Juhi…[et al.]. Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii. Enzyme Research No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-483858
American Medical Association (AMA)
Sikarwar, Juhi& Kaushik, Sanket& Sinha, Mau& Kaur, Punit& Sharma, Sujata& Singh, Tej P.. Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-483858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483858