Validation of the Antidiabetic and Hypolipidemic Effects of Hawthorn by Assessment of Gluconeogenesis and Lipogenesis Related Genes and AMP-Activated Protein Kinase Phosphorylation
Joint Authors
Lin, Yih-Jiun
Shih, Chun-Ching
Lin, Cheng-Hsiu
Wu, Jin-Bin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Since with the increased use of antidiabetic and antihyperlipidemic effect of phytonutrients for daily supplement has gained considerable attention worldwide, we examine the effect and molecular mechanism of Crataegus pinnatifida Bge.
var.
major N.E.
Br.
(hawthorn) by quantifying the expression of hepatic gluconeogenesis and lipogenesis on diabetes and dyslipidemia in high-fat (HF)-fed C57BL/6J mice.
Firstly, mice were divided randomly into two groups: the control (CON) group was fed with a low-fat diet, whereas the experimental group was fed a 45% HF diet for 8 weeks.
Afterwards, the CON group was treated with vehicle, whereas the HF group was subdivided into five groups and was given orally hawthorn extract (including 0.2, 0.5, 1.0 g/kg/day extracts) or rosiglitazone (Rosi) or vehicle for 4 weeks afterward.
Diabetic mice showed an increase in plasma glucose and insulin.
Glucose lowering was comparable with Rosi-treated mice.
This study demonstrated that hawthorn was effective in ameliorating the HF diet-induced hyperglycemia, hypertriglyceridemia and hypercholesterolaemia.
Hawthorn extract significantly increases the hepatic protein contents of AMP-activated protein kinase (AMPK) phosphorylation and reduces expression of phosphenol pyruvate carboxykinase (PEPCK) and glucose production.
Furthermore, hawthorn decreased in hepatic triacylglycerol and cholesterol synthesis (including sterol regulatory element binding protein-1c (SREBP-1c), fatty acid synthase (FAS), SREBP2).
An increase in expressions of apoA-I gene and high-density lipoprotein cholesterol (HDL-C) was detected in HF-fed mice treated with high dose hawthorn.
Our data suggest that hawthorn extract are capable of decreasing glucose production and triacylglycerol synthesis by inducing AMPK-phosphorylation and hawthorn is a candidate source of antidiabetic and antihyperlipidemic phytonutrients factors.
American Psychological Association (APA)
Shih, Chun-Ching& Lin, Cheng-Hsiu& Lin, Yih-Jiun& Wu, Jin-Bin. 2013. Validation of the Antidiabetic and Hypolipidemic Effects of Hawthorn by Assessment of Gluconeogenesis and Lipogenesis Related Genes and AMP-Activated Protein Kinase Phosphorylation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-483860
Modern Language Association (MLA)
Shih, Chun-Ching…[et al.]. Validation of the Antidiabetic and Hypolipidemic Effects of Hawthorn by Assessment of Gluconeogenesis and Lipogenesis Related Genes and AMP-Activated Protein Kinase Phosphorylation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-483860
American Medical Association (AMA)
Shih, Chun-Ching& Lin, Cheng-Hsiu& Lin, Yih-Jiun& Wu, Jin-Bin. Validation of the Antidiabetic and Hypolipidemic Effects of Hawthorn by Assessment of Gluconeogenesis and Lipogenesis Related Genes and AMP-Activated Protein Kinase Phosphorylation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-483860
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483860