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Genome-Wide and Gene-Specific Epigenomic Platforms for Hepatocellular Carcinoma Biomarker Development Trials
Joint Authors
Valle, Blanca
Michailidi, Christina
Torbenson, Michael
Meltzer, Stephen J.
Esteller, Manel
Brait, Mariana
Ili-Gangas, Carmen
Maldonado, Leonel
Perez, Jimena
Guerrero-Preston, Rafael
Kim, Myoung Sook
Brebi-Mieville, Priscilla
Soudry, Ethan
Sidransky, David
Yang, Quiang
Zhong, Xiaoli
Jaffe, Andrew
Source
Gastroenterology Research and Practice
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The majority of the epigenomic reports in hepatocellular carcinoma have focused on identifying novel differentially methylated drivers or passengers of the oncogenic process.
Few reports have considered the technologies in place for clinical translation of newly identified biomarkers.
The aim of this study was to identify epigenomic technologies that need only a small number of samples to discriminate HCC from non-HCC tissue, a basic requirement for biomarker development trials.
To assess that potential, we used quantitative Methylation Specific PCR, oligonucleotide tiling arrays, and Methylation BeadChip assays.
Concurrent global DNA hypomethylation, gene-specific hypermethylation, and chromatin alterations were observed as a hallmark of HCC.
A global loss of promoter methylation was observed in HCC with the Illumina BeadChip assays and the Nimblegen oligonucleotide arrays.
HCC samples had lower median methylation peak scores and a reduced number of significant promoter-wide methylated probes.
Promoter hypermethylation of RASSF1A, SSBP2, and B4GALT1 quantified by qMSP had a sensitivity ranging from 38% to 52%, a specificity of 100%, and an AUC from 0.58 to 0.75.
A panel combining these genes with HCC risk factors had a sensitivity of 87%, a specificity of 100%, and an AUC of 0.91.
American Psychological Association (APA)
Michailidi, Christina& Soudry, Ethan& Brait, Mariana& Maldonado, Leonel& Jaffe, Andrew& Ili-Gangas, Carmen…[et al.]. 2014. Genome-Wide and Gene-Specific Epigenomic Platforms for Hepatocellular Carcinoma Biomarker Development Trials. Gastroenterology Research and Practice،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-483871
Modern Language Association (MLA)
Michailidi, Christina…[et al.]. Genome-Wide and Gene-Specific Epigenomic Platforms for Hepatocellular Carcinoma Biomarker Development Trials. Gastroenterology Research and Practice No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-483871
American Medical Association (AMA)
Michailidi, Christina& Soudry, Ethan& Brait, Mariana& Maldonado, Leonel& Jaffe, Andrew& Ili-Gangas, Carmen…[et al.]. Genome-Wide and Gene-Specific Epigenomic Platforms for Hepatocellular Carcinoma Biomarker Development Trials. Gastroenterology Research and Practice. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-483871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483871