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Metabolic Control Analysis : A Tool for Designing Strategies to Manipulate Metabolic Pathways
Joint Authors
Moreno-Sánchez, Rafael
Saavedra, Emma
Rodríguez-Enríquez, Sara
Olín-Sandoval, Viridiana
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-30, 30 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-07-08
Country of Publication
Egypt
No. of Pages
30
Main Subjects
Abstract EN
The traditional experimental approaches used for changing the flux or the concentration of a particular metabolite of a metabolic pathway have been mostly based on the inhibition or over-expression of the presumed rate-limiting step.
However, the attempts to manipulate a metabolic pathway by following such approach have proved to be unsuccessful.
Metabolic Control Analysis (MCA) establishes how to determine, quantitatively, the degree of control that a given enzyme exerts on flux and on the concentration of metabolites, thus substituting the intuitive, qualitative concept of rate limiting step.
Moreover, MCA helps to understand (i) the underlying mechanisms by which a given enzyme exerts high or low control and (ii) why the control of the pathway is shared by several pathway enzymes and transporters.
By applying MCA it is possible to identify the steps that should be modified to achieve a successful alteration of flux or metabolite concentration in pathways of biotechnological (e.g., large scale metabolite production) or clinical relevance (e.g., drug therapy).
The different MCA experimental approaches developed for the determination of the flux-control distribution in several pathways are described.
Full understanding of the pathway properties when working under a variety of conditions can help to attain a successful manipulation of flux and metabolite concentration.
American Psychological Association (APA)
Moreno-Sánchez, Rafael& Saavedra, Emma& Rodríguez-Enríquez, Sara& Olín-Sandoval, Viridiana. 2008. Metabolic Control Analysis : A Tool for Designing Strategies to Manipulate Metabolic Pathways. Journal of Biomedicine and Biotechnology،Vol. 2008, no. 2008, pp.1-30.
https://search.emarefa.net/detail/BIM-483951
Modern Language Association (MLA)
Moreno-Sánchez, Rafael…[et al.]. Metabolic Control Analysis : A Tool for Designing Strategies to Manipulate Metabolic Pathways. Journal of Biomedicine and Biotechnology No. 2008 (2008), pp.1-30.
https://search.emarefa.net/detail/BIM-483951
American Medical Association (AMA)
Moreno-Sánchez, Rafael& Saavedra, Emma& Rodríguez-Enríquez, Sara& Olín-Sandoval, Viridiana. Metabolic Control Analysis : A Tool for Designing Strategies to Manipulate Metabolic Pathways. Journal of Biomedicine and Biotechnology. 2008. Vol. 2008, no. 2008, pp.1-30.
https://search.emarefa.net/detail/BIM-483951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483951