Human Chondrosarcoma Cells Acquire an Epithelial-Like Gene Expression Pattern via an Epigenetic Switch : Evidence for Mesenchymal-Epithelial Transition during Sarcomagenesis
Joint Authors
Provenzano, Matthew J.
Fitzgerald, Matthew P.
Gourronc, Francoise
Case, Adam J.
Domann, Frederick E.
Martin, James A.
Teoh, Melissa L. T.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Chondrocytes are mesenchymally derived cells that reportedly acquire some epithelial characteristics; however, whether this is a progression through a mesenchymal to epithelial transition (MET) during chondrosarcoma development is still a matter of investigation.
We observed that chondrosarcoma cells acquired the expression of four epithelial markers, E-cadherin,desmocollin 3, maspin, and 14-3-3σ, all of which are governed epigenetically through cytosine methylation.
Indeed, loss of cytosine methylation was tightly associated with acquired expression of both maspin and 14-3-3σ in chondrosarcomas.
In contrast, chondrocyte cells were negative for maspin and 14-3-3σ and displayed nearly complete DNA methylation.
Robust activation of these genes was also observed in chondrocyte cells following 5-aza-dC treatment.
We also examined the transcription factor snail which has been reported to be an important mediator of epithelial to mesenchymal transitions (EMTs).
In chondrosarcoma cells snail is downregulated suggesting a role for loss of snail expression in lineage maintenance.
Taken together, these results document an epigenetic switch associated with an MET-like phenomenon that accompanies chondrosarcoma progression.
American Psychological Association (APA)
Fitzgerald, Matthew P.& Gourronc, Francoise& Teoh, Melissa L. T.& Provenzano, Matthew J.& Case, Adam J.& Martin, James A.…[et al.]. 2011. Human Chondrosarcoma Cells Acquire an Epithelial-Like Gene Expression Pattern via an Epigenetic Switch : Evidence for Mesenchymal-Epithelial Transition during Sarcomagenesis. Sarcoma،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-483987
Modern Language Association (MLA)
Fitzgerald, Matthew P.…[et al.]. Human Chondrosarcoma Cells Acquire an Epithelial-Like Gene Expression Pattern via an Epigenetic Switch : Evidence for Mesenchymal-Epithelial Transition during Sarcomagenesis. Sarcoma No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-483987
American Medical Association (AMA)
Fitzgerald, Matthew P.& Gourronc, Francoise& Teoh, Melissa L. T.& Provenzano, Matthew J.& Case, Adam J.& Martin, James A.…[et al.]. Human Chondrosarcoma Cells Acquire an Epithelial-Like Gene Expression Pattern via an Epigenetic Switch : Evidence for Mesenchymal-Epithelial Transition during Sarcomagenesis. Sarcoma. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-483987
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-483987