Use of BODIPY (493503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
Joint Authors
Pratt, Stephen J. P.
Wohlers, Lindsay M.
Lovering, Richard M.
Spangenburg, Espen E.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs).
Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs.
We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal muscle and cultured adult single fibers.
Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization.
We believe that the use of the BODIPY (493/503) dye is an acceptable alternative and, under certain conditions, a simpler method for visualizing LDs stored within skeletal muscle.
American Psychological Association (APA)
Spangenburg, Espen E.& Pratt, Stephen J. P.& Wohlers, Lindsay M.& Lovering, Richard M.. 2011. Use of BODIPY (493503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-484000
Modern Language Association (MLA)
Spangenburg, Espen E.…[et al.]. Use of BODIPY (493503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-484000
American Medical Association (AMA)
Spangenburg, Espen E.& Pratt, Stephen J. P.& Wohlers, Lindsay M.& Lovering, Richard M.. Use of BODIPY (493503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-484000
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484000
