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Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis
Joint Authors
Imanishi, Yuji
Yaoita, Eishin
Ueda, Yoshihiko
Koda, Ryo
Yoshino, Atsunori
Takeda, Tetsuro
Kazama, Junichiro James
Kawamoto, Shinya
Narita, Ichiei
Source
International Journal of Nephrology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The origin of crescent forming cells in human glomerulonephritis (GN) remains unknown.
Some animal studies demonstrated that parietal epithelial cells of Bowman’s capsule (PECs) were the main component of proliferating cells and PEC-specific tight junction protein claudin-1 was expressed in crescentic lesions.
We investigated the expression of claudin-1 in human GN.
Immunohistochemistry for claudin-1 was performed on 17 kidney biopsy samples with crescent formation.
Colocalization of claudin-1 with intracellular tight junction protein ZO-1 was also evaluated by immunofluorescence double staining.
Claudin-1 is expressed mainly at the cell to cell contact site of proliferating cells in cellular crescentic lesions in patients with these forms of human GN.
Small numbers of crescent forming cells showed extrajunctional localization of claudin-1.
Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells.
In control samples, staining of claudin-1 was positive in PECs, but not in podocytes.
Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1.
Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman’s space.
American Psychological Association (APA)
Koda, Ryo& Yoshino, Atsunori& Imanishi, Yuji& Kawamoto, Shinya& Ueda, Yoshihiko& Yaoita, Eishin…[et al.]. 2014. Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis. International Journal of Nephrology،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-484027
Modern Language Association (MLA)
Koda, Ryo…[et al.]. Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis. International Journal of Nephrology No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-484027
American Medical Association (AMA)
Koda, Ryo& Yoshino, Atsunori& Imanishi, Yuji& Kawamoto, Shinya& Ueda, Yoshihiko& Yaoita, Eishin…[et al.]. Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis. International Journal of Nephrology. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-484027
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484027