Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2M Cell Cycle Arrest
Joint Authors
Sae-lim, Phorntip
Patmasiriwat, Pimpicha
Domann, Frederick E.
Suksamrarn, Apichart
Duangmano, Suwit
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular mechanism of radiosensitization if any.
Materials and Methods.
Human breast carcinoma cell lines were treated with cucurbitacin B before irradiation with 0–10 Gy of C137s gamma rays.
The effect of cucurbitacin B on cell-survival following irradiation was evaluated by colony-forming assay.
Cell cycle distributions were investigated using flow cytometry.
Real-time PCR and western blots were performed to investigate the expression of cell cycle checkpoints.
Results.
Cucurbitacin B inhibited breast cancer cell proliferation in a dose-dependent manner.
Only MDA-MB-231 and MCF7:5C cells but not SKBR-3 cells were radiosensitized by cucurbitacin B.
Flow cytometric analysis for DNA content indicated that cucurbitacin B resulted in G2/M arrest in MDA-MB-231 and MCF7:5C but not SKBR-3 cells.
Moreover, Real-time PCR and western blot analysis demonstrated upregulated p21 expression before irradiation, a likely cause of the cell cycle arrest.
Conclusion.
Taken together, these findings suggest that cucurbitacin B causes radiosensitization of some breast cancer cells, and that cucurbitacin B induced G2/M arrest is an important mechanism.
Therefore, combinations of cucurbitacin B with radiotherapy may be appropriate for experimental breast cancer treatment.
American Psychological Association (APA)
Duangmano, Suwit& Sae-lim, Phorntip& Suksamrarn, Apichart& Patmasiriwat, Pimpicha& Domann, Frederick E.. 2012. Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2M Cell Cycle Arrest. Journal of Oncology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484126
Modern Language Association (MLA)
Duangmano, Suwit…[et al.]. Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2M Cell Cycle Arrest. Journal of Oncology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-484126
American Medical Association (AMA)
Duangmano, Suwit& Sae-lim, Phorntip& Suksamrarn, Apichart& Patmasiriwat, Pimpicha& Domann, Frederick E.. Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2M Cell Cycle Arrest. Journal of Oncology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484126
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484126