![](/images/graphics-bg.png)
Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia
Joint Authors
Tong, Yigang
Huang, Yong
Peng, Fan
Fan, Hang
Yuan, Shoujun
Chen, Chen
Fan, Huahao
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The advent of methicillin-resistant Staphylococcus aureus (MRSA) and the frequent and excessive abuse of ventilators have made MRSA pneumonia an inordinate threat to human health.
Appropriate antibacterial therapies are crucial, including the use of lysostaphin as an alternative to antibiotics.
To explore the potential use of lysostaphin as a therapeutic agent for MRSA pneumonia, mice were intranasally infected with MRSA and then treated with recombinant lysostaphin (rLys; 45 mg/kg in the high-dose group and 1 mg/kg in the low-dose group) (0.33 mg/mL, 15 mg/mL), vancomycin (120 mg/kg) (40 mg/mL), or phosphate-buffered saline (PBS, negative control) 4 h after infection.
Therapeutic efficacy was assessed by mouse survival, lung histopathology, bacterial density in the lungs, bodyweight, lung weight, temperature, white blood cells counts, lymphocytes counts, granulocytes counts, and monocytes counts.
The mice treated with rLys showed lower mortality, less lung parenchymal damage, and lower bacterial density at metastatic tissue sites than mice treated with PBS or vancomycin.
The overall mortality was 100%, 60%, 40%, and 60% for the control, vancomycin, high-dose rLys, and low-dose rLys groups, respectively.
These findings indicate that, as a therapeutic agent for MRSA pneumonia, lysostaphin exerts profound protective effects in mice against the morbidity and mortality associated with S.
aureus pneumonia.
American Psychological Association (APA)
Chen, Chen& Fan, Huahao& Huang, Yong& Peng, Fan& Fan, Hang& Yuan, Shoujun…[et al.]. 2014. Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484169
Modern Language Association (MLA)
Chen, Chen…[et al.]. Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-484169
American Medical Association (AMA)
Chen, Chen& Fan, Huahao& Huang, Yong& Peng, Fan& Fan, Hang& Yuan, Shoujun…[et al.]. Recombinant Lysostaphin Protects Mice from Methicillin-Resistant Staphylococcus aureus Pneumonia. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-484169
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484169