Selective Detection of NADPH Oxidase in Polymorphonuclear Cells by Means of NAD(P)H-Based Fluorescence Lifetime Imaging
Joint Authors
Andresen, V.
Niesner, R.
Narang, P.
Gericke, K.-H.
Spiecker, H.
Gunzer, M.
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-11-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
NADPH oxidase (NOX2) is a multisubunit membrane-bound enzyme complex that, upon assembly in activated cells, catalyses the reduction of free oxygen to its superoxide anion, which further leads to reactive oxygen species (ROS) that are toxic to invading pathogens, for example, the fungus Aspergillus fumigatus.
Polymorphonuclear cells (PMNs) employ both nonoxidative and oxidative mechanisms to clear this fungus from the lung.
The oxidative mechanisms mainly depend on the proper assembly and function of NOX2.
We identified for the first time the NAD(P)H-dependent enzymes involved in such oxidative mechanisms by means of biexponential NAD(P)H-fluorescence lifetime imaging (FLIM).
A specific fluorescence lifetime of 3670±140 picoseconds as compared to 1870 picoseconds for NAD(P)H bound to mitochondrial enzymes could be associated with NADPH bound to oxidative enzymes in activated PMNs.
Due to its predominance in PMNs and due to the use of selective activators and inhibitors, we strongly believe that this specific lifetime mainly originates from NOX2.
Our experiments also revealed the high site specificity of the NOX2 assembly and, thus, of the ROS production as well as the dynamic nature of these phenomena.
On the example of NADPH oxidase, we demonstrate the potential of NAD(P)H-based FLIM in selectively investigating enzymes during their cellular function.
American Psychological Association (APA)
Niesner, R.& Narang, P.& Spiecker, H.& Andresen, V.& Gericke, K.-H.& Gunzer, M.. 2008. Selective Detection of NADPH Oxidase in Polymorphonuclear Cells by Means of NAD(P)H-Based Fluorescence Lifetime Imaging. Journal of Biophysics،Vol. 2008, no. 2008, pp.1-13.
https://search.emarefa.net/detail/BIM-484198
Modern Language Association (MLA)
Niesner, R.…[et al.]. Selective Detection of NADPH Oxidase in Polymorphonuclear Cells by Means of NAD(P)H-Based Fluorescence Lifetime Imaging. Journal of Biophysics No. 2008 (2008), pp.1-13.
https://search.emarefa.net/detail/BIM-484198
American Medical Association (AMA)
Niesner, R.& Narang, P.& Spiecker, H.& Andresen, V.& Gericke, K.-H.& Gunzer, M.. Selective Detection of NADPH Oxidase in Polymorphonuclear Cells by Means of NAD(P)H-Based Fluorescence Lifetime Imaging. Journal of Biophysics. 2008. Vol. 2008, no. 2008, pp.1-13.
https://search.emarefa.net/detail/BIM-484198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484198