Rational Design of a Triple Reporter Gene for Multimodality Molecular Imaging
Joint Authors
Chen, Fu-Du
Hwu, Luen
Chen, Ran-Chou
Lin, Kang-Ping
Ke, Chien-Chih
Hsieh, Ya-Ju
Yeh, Skye Hsin-Hsien
Lin, Chien-Feng
Liu, Ren-Shyan
Wang, Hsin-Ell
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Multimodality imaging using noncytotoxic triple fusion (TF) reporter genes is an important application for cell-based tracking, drug screening, and therapy.
The firefly luciferase (fl), monomeric red fluorescence protein (mrfp), and truncated herpes simplex virus type 1 thymidine kinase SR39 mutant (ttksr39) were fused together to create TF reporter gene constructs with different order.
The enzymatic activities of TF protein in vitro and in vivo were determined by luciferase reporter assay, H-FEAU cellular uptake experiment, bioluminescence imaging, and micropositron emission tomography (microPET).
The TF construct expressed in H1299 cells possesses luciferase activity and red fluorescence.
The tTKSR39 activity is preserved in TF protein and mediates high levels of H-FEAU accumulation and significant cell death from ganciclovir (GCV) prodrug activation.
In living animals, the luciferase and tTKSR39 activities of TF protein have also been successfully validated by multimodality imaging systems.
The red fluorescence signal is relatively weak for in vivo imaging but may expedite FACS-based selection of TF reporter expressing cells.
We have developed an optimized triple fusion reporter construct DsRedm-fl-ttksr39 for more effective and sensitive in vivo animal imaging using fluorescence, bioluminescence, and PET imaging modalities, which may facilitate different fields of biomedical research and applications.
American Psychological Association (APA)
Hsieh, Ya-Ju& Hwu, Luen& Ke, Chien-Chih& Yeh, Skye Hsin-Hsien& Lin, Chien-Feng& Chen, Fu-Du…[et al.]. 2014. Rational Design of a Triple Reporter Gene for Multimodality Molecular Imaging. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-484476
Modern Language Association (MLA)
Hsieh, Ya-Ju…[et al.]. Rational Design of a Triple Reporter Gene for Multimodality Molecular Imaging. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-484476
American Medical Association (AMA)
Hsieh, Ya-Ju& Hwu, Luen& Ke, Chien-Chih& Yeh, Skye Hsin-Hsien& Lin, Chien-Feng& Chen, Fu-Du…[et al.]. Rational Design of a Triple Reporter Gene for Multimodality Molecular Imaging. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-484476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484476