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An Assessment of Whole Blood and Fractions by Nested PCR as a DNA Source for Diagnosing Canine Ehrlichiosis and Anaplasmosis
Joint Authors
Lustosa, Elane Maria Camboim
Camboim, Expedito Kennedy Alves
Rotondano, Tereza Emmanuelle de Farias
de Andrade, Paulo Paes
de Melo, Marcia Almeida
Cordeiro, Aline Antas
de Almeida, Alzira Maria Paiva
de Azevedo, Sérgio Santos
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Ehrlichiosis and anaplasmosis are tick-borne diseases.
Ehrlichia canis and Anaplasma platys infect mainly white cells and platelets, respectively.
The main DNA source for PCR is peripheral blood, but the potential of blood cell fractions has not been extensively investigated.
This study aims at assessment of whole blood (WB) and blood fractions potential in nested PCR (nPCR) to diagnose canine ehrlichiosis and anaplasmosis.
The 16S rRNA gene was amplified in 71.4, 17.8, 31.57, and 30% of the WB, granulocyte (G), mononuclear cells (M), and buffy coat (BC) samples.
Compared to the WB, the sensitivity of the PCR was 42.86% for the M, and BC fractions, 21.43% for the G, and 33.33% for the blood clot (C).
There was fair agreement between the WB and M, BC and C, and slight with the G.
Fair agreement occurred between the nPCR and morulae in the blood smear.
One animal was coinfected with A.
platys and E.
canis.
This study provided the first evidence of A.
platys infection in dogs in Paraíba, Brazil, and demonstrated that WB is a better DNA source than blood fractions to detect Ehrlichia and Anaplasma by nPCR, probably because of the plasma bacterial concentration following host cell lysis.
American Psychological Association (APA)
Rotondano, Tereza Emmanuelle de Farias& de Almeida, Alzira Maria Paiva& Lustosa, Elane Maria Camboim& Cordeiro, Aline Antas& Camboim, Expedito Kennedy Alves& de Azevedo, Sérgio Santos…[et al.]. 2012. An Assessment of Whole Blood and Fractions by Nested PCR as a DNA Source for Diagnosing Canine Ehrlichiosis and Anaplasmosis. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-484505
Modern Language Association (MLA)
Rotondano, Tereza Emmanuelle de Farias…[et al.]. An Assessment of Whole Blood and Fractions by Nested PCR as a DNA Source for Diagnosing Canine Ehrlichiosis and Anaplasmosis. The Scientific World Journal No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-484505
American Medical Association (AMA)
Rotondano, Tereza Emmanuelle de Farias& de Almeida, Alzira Maria Paiva& Lustosa, Elane Maria Camboim& Cordeiro, Aline Antas& Camboim, Expedito Kennedy Alves& de Azevedo, Sérgio Santos…[et al.]. An Assessment of Whole Blood and Fractions by Nested PCR as a DNA Source for Diagnosing Canine Ehrlichiosis and Anaplasmosis. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-484505
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484505