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Efficient Degradation of Feather by Keratinase Producing Bacillus sp.
Joint Authors
Kumari Chitturi, Ch. M.
Jeevana Lakshmi, P.
Lakshmi, V. V.
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Keratinase producing microorganisms are being increasingly utilized for degradation and recycling of poultry feather waste.
Two native strains BF11 (Bacillus subtilis) and BF21 (Bacillus cereus) degrading keratin completely were characterized.
The native strains produced more than 10 KU/mL of enzyme.
Strain improvement resulted in isolation of MBF11 and MBF21 from BF11 and BF21 isolates, respectively.
Optimization of nutritional and physical parameters of these MBF isolates at laboratory scale increased the overall keratinase activity by 50-fold resulting in a yield of 518–520 KU/mL.
Fermentation media designed with starch as carbon source and soya bean meal as nitrogen source supported high levels of enzyme production.
The optimum conditions for enzyme production were determined to be pH 8.5 and temperatures of 45–55°C for MBF11 and 37°C for MBF21, respectively.
Culture filtrate showed a significant increase in the amounts of cysteine, cystine, methionine, and total free amino acids during the fermentation period.
The ratio of organic sulphur concentration was also considerably higher than that of the inorganic sulphate in the culture filtrate suggesting the hydrolysis of disulphide by the isolates.
American Psychological Association (APA)
Jeevana Lakshmi, P.& Kumari Chitturi, Ch. M.& Lakshmi, V. V.. 2013. Efficient Degradation of Feather by Keratinase Producing Bacillus sp.. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-484732
Modern Language Association (MLA)
Jeevana Lakshmi, P.…[et al.]. Efficient Degradation of Feather by Keratinase Producing Bacillus sp.. International Journal of Microbiology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-484732
American Medical Association (AMA)
Jeevana Lakshmi, P.& Kumari Chitturi, Ch. M.& Lakshmi, V. V.. Efficient Degradation of Feather by Keratinase Producing Bacillus sp.. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-484732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484732