The Bridge Helix of RNA Polymerase Acts as a Central Nanomechanical Switchboard for Coordinating Catalysis and Substrate Movement
Author
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The availability of in vitro assembly systems to produce recombinant archaeal RNA polymerases (RNAPs) offers one of the most powerful experimental tools for investigating the still relatively poorly understood molecular mechanisms underlying RNAP function.
Over the last few years, we pioneered new robot-based high-throughput mutagenesis approaches to study structure/function relationships within various domains surrounding the catalytic center.
The Bridge Helix domain, which appears in numerous X-ray structures as a 35-amino-acid-long alpha helix, coordinates the concerted movement of several other domains during catalysis through kinking of two discrete molecular hinges.
Mutations affecting these kinking mechanisms have a direct effect on the specific catalytic activity of RNAP and can in some instances more than double it.
Molecular dynamics simulations have established themselves as exceptionally useful for providing additional insights and detailed models to explain the underlying structural motions.
American Psychological Association (APA)
Weinzierl, Robert O. J.. 2012. The Bridge Helix of RNA Polymerase Acts as a Central Nanomechanical Switchboard for Coordinating Catalysis and Substrate Movement. Archaea،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-484744
Modern Language Association (MLA)
Weinzierl, Robert O. J.. The Bridge Helix of RNA Polymerase Acts as a Central Nanomechanical Switchboard for Coordinating Catalysis and Substrate Movement. Archaea No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-484744
American Medical Association (AMA)
Weinzierl, Robert O. J.. The Bridge Helix of RNA Polymerase Acts as a Central Nanomechanical Switchboard for Coordinating Catalysis and Substrate Movement. Archaea. 2012. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-484744
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484744