CNPase Expression in Olfactory Ensheathing Cells
Joint Authors
Lankford, Karen L.
Sasaki, Masanori
Radtke, Christine
Gallo, Vittorio
Kocsis, Jeffery D.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A large body of work supports the proposal that transplantation of olfactory ensheathing cells (OECs) into nerve or spinal cord injuries can promote axonal regeneration and remyelination.
Yet, some investigators have questioned whether the transplanted OECs associate with axons and form peripheral myelin, or if they recruit endogenous Schwann cells that form myelin.
Olfactory bulbs from transgenic mice expressing the enhanced green fluorescent protein (eGFP) under the control of the 2-3-cyclic nucleotide 3-phosphodiesterase (CNPase) promoter were studied.
CNPase is expressed in myelin-forming cells throughout their lineage.
We examined CNPase expression in both in situ in the olfactory bulb and in vitro to determine if OECs express CNPase commensurate with their myelination potential.
eGFP was observed in the outer nerve layer of the olfactory bulb.
Dissociated OECs maintained in culture had both intense eGFP expression and CNPase immunostaining.
Transplantation of OECs into transected peripheral nerve longitudinally associated with the regenerated axons.
These data indicate that OECs in the outer nerve layer of the olfactory bulb of CNPase transgenic mice express CNPase.
Thus, while OECs do not normally form myelin on olfactory nerve axons, their expression of CNPase is commensurate with their potential to form myelin when transplanted into injured peripheral nerve.
American Psychological Association (APA)
Radtke, Christine& Sasaki, Masanori& Lankford, Karen L.& Gallo, Vittorio& Kocsis, Jeffery D.. 2011. CNPase Expression in Olfactory Ensheathing Cells. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-484751
Modern Language Association (MLA)
Radtke, Christine…[et al.]. CNPase Expression in Olfactory Ensheathing Cells. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-484751
American Medical Association (AMA)
Radtke, Christine& Sasaki, Masanori& Lankford, Karen L.& Gallo, Vittorio& Kocsis, Jeffery D.. CNPase Expression in Olfactory Ensheathing Cells. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-484751
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484751