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Improved Chromatographic Methods for Determination of Bioactive Compounds from Aloe vera Leaves
Joint Authors
Liazid, A.
Barbero, G. F.
Azaroual, L.
Barroso, C. G.
Brigui, J.
Palma, M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In this work three chromatographic methods were developed to reduce the total time of the analysis of main compounds in Aloe vera extracts.
The first method was developed in a regular reverse phase chromatographic system using a particulate reverse phase C-18 column.
Methods already published were used as a starting point for the development of the new method.
All the compounds were separated in 32 minutes.
The second method was developed in a regular reverse phase chromatographic system employing a monolithic type column.
Using a 4.5 mL min−1 flow, the total time of analysis was reduced to 6 minutes with very similar resolution values.
The third method was developed in an ultraperformance liquid chromatographic system, and the final time for the analysis of the phenolic compounds was reduced to 4 minutes.
The analytical properties of the three chromatographic methods were compared for the main compounds in the chromatograms.
Robustness of the three new methods was also checked with regard to the injection volume and the amount of methanol in the sample.
A fast method (4 min) is then available for bioactive compounds from Aloe vera determination.
American Psychological Association (APA)
Azaroual, L.& Liazid, A.& Barbero, G. F.& Brigui, J.& Palma, M.& Barroso, C. G.. 2012. Improved Chromatographic Methods for Determination of Bioactive Compounds from Aloe vera Leaves. ISRN Chromatography،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-484806
Modern Language Association (MLA)
Azaroual, L.…[et al.]. Improved Chromatographic Methods for Determination of Bioactive Compounds from Aloe vera Leaves. ISRN Chromatography No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-484806
American Medical Association (AMA)
Azaroual, L.& Liazid, A.& Barbero, G. F.& Brigui, J.& Palma, M.& Barroso, C. G.. Improved Chromatographic Methods for Determination of Bioactive Compounds from Aloe vera Leaves. ISRN Chromatography. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-484806
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484806