Selection of a MCF-7 Breast Cancer Cell Subpopulation with High Sensitivity to IL-1β : Characterization of and Correlation between Morphological and Molecular Changes Leading to Increased Invasiveness
Joint Authors
Pérez-Yépez, Eloy Andres
Ayala-Sumuano, Jorge-Tonatiuh
Reveles-Espinoza, Alicia Maria
Meza, Isaura
Source
International Journal of Breast Cancer
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Cancer and inflammation are closely related in tumor malignancy prognosis.
Breast cancer MCF-7 cells have a poor invasive phenotype, although, under IL-1β stimulus, acquire invasive features.
Cell response heterogeneity has precluded precise evaluation of the malignant transition.
MCF-7A3 cells were selected for high sensitivity to IL-1β stimulus, uniform expression of CXCR4, and stability of IL1-RI.
Structural changes, colony formation ability, proliferation rate, chemotaxis, Matrigel invasion, E-cadherin mRNA expression and protein localization were determined in these cells and in MCF-7 parental cells under the stimulus of IL-1β.
Selected MCF-7A3 cells showed a uniform response to IL-1β stimulation increasing features of invasive cells such as scattering, colony formation, proliferation, chemokinesis and invasion.
Basal expression of E-cadherin mRNA was higher, and IL-1β stimulus had no further effect at early times of cytokine exposure.
Total E-cadherin levels remained unchanged in parental cells, whereas levels decreased, as MCF-7A3 cells became fibroblastoid or scattered.
Triton X-100 soluble/insoluble E-cadherin ratios were highly increased in these cells, while, in MCF-7pl cells, ratios could not be correlated with morphology changes.
MCF-7A3 cells uniform response to IL-1β allowed characterization of changes induced by the cytokine that had not been assessed when using heterogeneous cell lines.
American Psychological Association (APA)
Pérez-Yépez, Eloy Andres& Ayala-Sumuano, Jorge-Tonatiuh& Reveles-Espinoza, Alicia Maria& Meza, Isaura. 2012. Selection of a MCF-7 Breast Cancer Cell Subpopulation with High Sensitivity to IL-1β : Characterization of and Correlation between Morphological and Molecular Changes Leading to Increased Invasiveness. International Journal of Breast Cancer،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-484810
Modern Language Association (MLA)
Pérez-Yépez, Eloy Andres…[et al.]. Selection of a MCF-7 Breast Cancer Cell Subpopulation with High Sensitivity to IL-1β : Characterization of and Correlation between Morphological and Molecular Changes Leading to Increased Invasiveness. International Journal of Breast Cancer No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-484810
American Medical Association (AMA)
Pérez-Yépez, Eloy Andres& Ayala-Sumuano, Jorge-Tonatiuh& Reveles-Espinoza, Alicia Maria& Meza, Isaura. Selection of a MCF-7 Breast Cancer Cell Subpopulation with High Sensitivity to IL-1β : Characterization of and Correlation between Morphological and Molecular Changes Leading to Increased Invasiveness. International Journal of Breast Cancer. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-484810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484810