Simultaneous Determination of Six Major Constituents of the Herbal Formula Insampaedok-san Using HPLC-PDA

Abstract EN

A simple, rapid, and accurate high-performance liquid chromatographic method was applied to the quantitative analysis of six components of a traditional herbal formulation, Insampaedok-san (ISPDS): liquiritin (1), ferulic acid (2), naringin (3), hesperidin (4), neohesperidin (5), and glycyrrhizin (6).

The six components were separated within 35 min using a Gemini C18 column maintained at 40°C.

The mobile phase was composed of 1.0% (v/v) aqueous acetic acid (A) and 1.0% (v/v) acetic acid in acetonitrile (B) by gradient elution.

The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 254, 280, and 320 nm.

The calibration curves showed good linearity (R2=1.0000) for different concentration ranges.

The recovery of each component was in the range of 92.62%–105.96%, with a relative standard deviation (RSD) of less than 4.0%.

The RSDs for intra- and interday precision were 0.04%–1.70% and 0.06%–2.56%, respectively.

The concentration of each of the six components of ISPDS was in the range 0.72–9.88 mg g−1.