DUSP1 Gene Polymorphisms Are Associated with Obesity-Related Metabolic Complications among Severely Obese Patients and Impact on Gene Methylation and Expression
Joint Authors
Bouchard, L.
Hould, Frédéric-Simon
Guénard, Frédéric
Vohl, Marie-Claude
Marceau, Picard
Tchernof, André
Lebel, Stéfane
Pérusse, L.
Deshaies, Yves
Source
International Journal of Genomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The DUSP1 gene encodes a member of the dual-specificity phosphatase family previously identified as being differentially expressed in visceral adipose tissue (VAT) of severely obese men with versus without the metabolic syndrome.
Objective.
To test the association between DUSP1 polymorphisms, obesity-related metabolic complications, gene methylation, and expression levels in VAT.
Methods.
The DUSP1 locus and promoter region were sequenced in 25 individuals.
SNPs were tested for association with obesity-related complications in a cohort of more than 1900 severely obese individuals.
The impact of SNPs on methylation levels of 36 CpG sites and correlations between DNA methylation and gene expression levels in VAT were computed in a subset of 14 samples.
Results.
Heterozygotes for rs881150 had lower HDL-cholesterol levels (HDL-C; P=0.01), and homozygotes for the minor allele of rs13184134 and rs7702178 had increased fasting glucose levels (P=0.04 and 0.01, resp.).
rs881150 was associated with methylation levels of CpG sites located ~1250 bp upstream the transcription start site.
Methylation levels of 4 CpG sites were inversely correlated with DUSP1 gene expression.
Conclusion.
These results suggest that DUSP1 polymorphisms modulate plasma glucose and HDL-C levels in obese patients possibly through alterations of DNA methylation and gene expression levels.
American Psychological Association (APA)
Guénard, Frédéric& Bouchard, L.& Tchernof, André& Deshaies, Yves& Hould, Frédéric-Simon& Lebel, Stéfane…[et al.]. 2013. DUSP1 Gene Polymorphisms Are Associated with Obesity-Related Metabolic Complications among Severely Obese Patients and Impact on Gene Methylation and Expression. International Journal of Genomics،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-484883
Modern Language Association (MLA)
Guénard, Frédéric…[et al.]. DUSP1 Gene Polymorphisms Are Associated with Obesity-Related Metabolic Complications among Severely Obese Patients and Impact on Gene Methylation and Expression. International Journal of Genomics No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-484883
American Medical Association (AMA)
Guénard, Frédéric& Bouchard, L.& Tchernof, André& Deshaies, Yves& Hould, Frédéric-Simon& Lebel, Stéfane…[et al.]. DUSP1 Gene Polymorphisms Are Associated with Obesity-Related Metabolic Complications among Severely Obese Patients and Impact on Gene Methylation and Expression. International Journal of Genomics. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-484883
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484883